Pharmaceutical and Biomedical Analysis Using Liquid Chromatography Coupled to Pre/Post Column DerivatizationParaskevas D. Tzanavaras*
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotelian University of Thessaloniki, GR-54124 Thessaloniki, Greece
- *Corresponding Author:
- Paraskevas D Tzanavaras
Laboratory of Analytical Chemistry
Department of Chemistry, Aristotelian University of Thessaloniki
GR- 54124 Thessaloniki, Greece
E-mail: [email protected]
Received date: October 22, 2012; Accepted date: October 24, 2012; Published date: October 26, 2012
Citation: Tzanavaras PD (2012) Pharmaceutical and Biomedical Analysis Using Liquid Chromatography Coupled to Pre/Post Column Derivatization. Pharmaceut Anal Acta 3:171. doi: 10.4172/2153-2435.1000171
Copyright: © 2012 Tzanavaras PD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Liquid chromatography (LC or HPLC) is the predominant technique in pharmaceutical and biomedical analysis. LC is fairly simple and the separation mechanism is rather straightforward and readily understandable compared to other more complicated separation techniques (e.g. capillary electrophoresis). A wide selection of specific and non-specific detectors and numerous analytical columns and stationary phases cover almost all possible applications. Furthermore, instrument manufacturers offer highly reliable instrumentation capable of automated operation on a 24-h basis.