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Abstract
Pharmaceutical and Biomedical Analysis Using Liquid Chromatography Coupled to Pre/Post Column Derivatization
Liquid chromatography (LC or HPLC) is the predominant technique in pharmaceutical and biomedical analysis. LC is fairly simple and the separation mechanism is rather straightforward and readily understandable compared to other more complicated separation techniques (e.g. capillary electrophoresis). A wide selection of specific and non-specific detectors and numerous analytical columns and stationary phases cover almost all possible applications. Furthermore, instrument manufacturers offer highly reliable instrumentation capable of automated operation on a 24-h basis.