Pharmacokinetic Evaluation of Metolazone Tablets using Healthy Human Volunteers
Basvan Babu*, Selvadurai Muralidharan, Subramaniya Nainar Meyyanathan and Bhojraj Suresh
Department of Pharmaceutical Analysis, J.S.S.College of Pharmacy, (Off Campus of JSS University, Mysore) Ootacamund, Rocklands, Ooty
- *Corresponding Author:
- Dr. Basvan Babu
Department of Pharmaceutical Analysis
J.S.S.College of pharmacy, Tamilnadu, India
Tel : +91-423- 2443393
Fax : +91-423-2442937
E-mail: [email protected]
Received Date: December 29, 2009; Accepted Date: January 21, 2010; Published Date: January 22, 2010
Citation: Babu B, Muralidharan S, Meyyanathan SN, Suresh B (2010) Pharmacokinetic Evaluation of Metolazone Tablets using Healthy Human Volunteers. J Bioequiv Availab 2: 015-017. doi: 10.4172/jbb.1000024
Copyright: © 2010 Babu B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A sensitive and reproducible high performance liquid chromatography (HPLC) method has been developed and validated for the quantification of metolazone in human plasma, after solid phase extraction (SPE). A Good resolution was achieved on a reverse-phase LichroCART Purospher® C18 column using the mobile phase acetonitrile– 0.5% triethylamine (35:65) in isocratic elution with a total run time of 15 min. The analyte, metolazone, was detected by using high performance liquid chromatography with the support of photo diode array detector. Limit of detection and Lower limit of quantification was found to be 1 and 2.5 ng/mL. The present method was successfully applied in the pharmacokinetic study of metolazone in human plasma.