alexa Pharmacokinetic Investigation of Main Bioactive Compone
ISSN: 2161-0444

Medicinal chemistry
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Research Article

Pharmacokinetic Investigation of Main Bioactive Components from Epimedium-Derived Flavonoids in Rabbit Serum by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

Liufang Hu1,2, Yuanru Zheng1,2, Yi Zhang1,2, Zifei Qin1,2,3*, Yi Dai1,2, Lin Qin4, Zhihong Yao1,2* and Xinsheng Yao1,2,3

1College of Pharmacy, Jinan University, Guangzhou 510632, China

2Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, PR China

3Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, PR China

4Musculoskeletal Research Laboratory, Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, Satin, NT Hong Kong SAR, PR China

*Corresponding Author:
Zhihong Yao
College of Pharmacy, Jinan University
Guangzhou 510632, PR China
Tel: 862085221767
Fax: 862085221559
E-mail: [email protected] (or) [email protected]
Zifei Qin
College of Pharmacy, Jinan University
Guangzhou 510632, PR China
Tel: 862085220010
E-mail: [email protected]

Received date: May 02, 2017; Accepted date: May 08, 2017; Published date: May 13, 2017

Citation: Hu L, Zheng Y, Zhang Y, Qin Z, Dai Y, et al. (2017) Pharmacokinetic Investigation of Main Bioactive Components from Epimedium-Derived Flavonoids in Rabbit Serum by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry. Med Chem (Los Angeles) 7:869-874. doi: 10.4172/2161-0444.1000444

Copyright: © 2017 Hu L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Total Epimedium-derived flavonoids (EF) were reported to be a group of major bioactive constituents present in genus Epimedium for the treatment of osteonecrosis. The present study aimed to investigate the pharmacokinetics of main bioactive components of EF in rabbit.

Methods: A rapid and sensitive method for the simultaneous determination of icariin, icariside I, icariside II and icaritin in serum was established and validated by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS). Serum samples were pretreated with water-saturated ethyl acetate. Furthermore, sample separation was achieved on an Waters BEH C18 column (50 mm × 2.1 mm, 1.7 μm) by gradient elution with acetonitrile-water (both including 0.1% formic acid) as mobile phase. Finally, pharmacokinetics of EF in rabbit serum was investigated.

Results: All calibration curves displayed excellent linearity (r2>0.99) within 1.2~24 ng/mL. The assay was specific, precise and accurate, as demonstrated with intra-run precisions not more than 12.6%, inter-run precisions less than 9.9% and accuracies between -4.4% and 9.3%. Meanwhile, the matrix effects, extraction recoveries and stabilities were all satisfactory. Moreover, this validated method was successfully applied to analyse the pharmacokinetic of EF in rabbit serum after oral dosage of 80 mg/kg.

Conclusion: These results would enlarge our knowledge about main bioactive components of EF for the treatment of osteonecrosis in rabbit.

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