Pharmacokinetic Study of Lappaconitine Hydrobromide Transfersomes in Rats by LC-MSZheng WS*, Sheng YX, Zhang YJ, Fang XQ and Wang LL
- *Corresponding Author:
- Wen-sheng Zheng
Institute of Materia Medica
Chinese Academy of Medical Sciences& Peking Union Medical College
Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulations
Beijing 100050, People’s Republic of China
Tel: +86 010 63165233
Fax: +86 010 63017757
E-mail: [email protected]
Received date: March 14, 2013; Accepted date: March 23, 2013; Published date: March 26, 2013
Citation: Zheng WS, Sheng YX, Zhang YJ, Fang XQ, Wang LL (2013) Pharmacokinetic Study of Lappaconitine Hydrobromide Transfersomes in Rats by LC-MS. Pharmaceut Anal Acta 4:217. doi: 10.4172/2153-2435.1000217
Copyright: © 2013 Zheng WS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determination of lappaconitine hydrobromide in rat plasma and study on the pharmacokinetics of lappaconitine hydrobromide transfersomes in rat. Analyses were performed on AltimaTM HP C18 column (50 mm×2.1 mm, 3 μm) with internal standard of tetrahydropalmatine and a mobile phase of methanol-0.1% formic acid (80:20). Agilent™ LC/ MSD QQQ mass spectrometer with the mode of multiple reactions monitoring (MRM) was applied in the detection. Pharmacokinetic parameters of isodose lappaconitine hydrobromide transfersomes in transdermal patch were analyzed and calculated by DASver 2.0 software. The results indicated that with the linear range of 2.0-2000.0 ng/ml, quantitative lower limit was 2.0 ng and both the inter-day and the intra-day precisions were less than 9.9%. Lappaconitine hydrobromide transfersomes could significantly increase AUC and extend the circulation time of in rats.