Pharmacokinetics of CasiopeÃÂna IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity
- *Corresponding Author:
- Lena Ruiz-Azuara, Ph.D. Facultad de Química.
Departamento de Química Inorgánica y Nuclear
Autónoma de México, 04510
Tel/Fax: (5255) 56223529
E-mail: [email protected]
Received Date: November 08, 2012; Accepted Date: December 17, 2012; Published Date: December 19, 2012
Citation: Cañas-Alonso RC, Fuentes-Noriega I, Ruiz-Azuara L (2010) Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity. J Bioanal Biomed 2:028-034. doi:10.4172/1948-593X.1000018
Copyright: © 2010 Cañas-Alonso RC, et al. This is an open-access articldistributed under the terms of the Creative Commons Attribution License, hich permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Casiopeína IIgly is a mixed chelate coordination complex with copper core that have demonstrated high antineoplas- tic activity in vitro and in vivo . In the present work, a de- veloped a nd va lida ted method for mea surement of Casiopeína IIgly in beagle dog and its application in a phar- macokinetic study is reported. The analyte was isolated from blood by solid-phase extraction using Strata X cartridges. The analysis was carried out on a Synergy Polar-RP col- umn (30 X 2.0 mm) using an isocratic elution and MeOH/ HFBA 0.1% (4:6) as the mobile phase. An Agilent LC/ MSD Trap VL equipped with an ionization electrospray source, was operated in selective ion storage (SIS) using stable ion [Cu (II) (F 7 C 3 COO) 4,7-dimethyl phen ] + with 484 m/z for quantification result of ESI reaction between Casiopeína IIgly and HFBA. The method demonstrated to be linear (r = 0.9992) in the range from the 0.1 to 15 μ g/ml with a limit of detection (LOD) of 50 ng/ml. All the param- eters of validation such as selectivity, accuracy, precision and recovery were within the required limits. Pharmacoki- netics assay was carried out at 2 doses, indicated a high elimination rate.