alexa Phenotypic and Genotypic Diversity of Rhizobia Nodulating Faba Bean from Various Egyptian Locations
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Phenotypic and Genotypic Diversity of Rhizobia Nodulating Faba Bean from Various Egyptian Locations

Omar A Hewedy*, Ragaa A Eissa, Elzanaty AM, Nagaty HH and Abd Elbary MI
Genetics Department, Faculty of Agriculture, Minoufiya University, Shibin El-Kom, Egypt
Corresponding Author : Omar A Hewedy
Genetics Department, Faculty of Agriculture
Minoufiya University, Shibin El-Kom, Egypt
Tel: 00201223570768
E-mail: [email protected]
Received June 23, 2014; Accepted July 21, 2014; Published July 30, 2014
Citation: Hewedy OA, Eissa RA, Elzanaty AM, Nagaty HH, Abd Elbary MI (2014) Phenotypic and Genotypic Diversity of Rhizobia Nodulating Faba Bean from Various Egyptian Locations. J Bioprocess Biotech 4:170. doi:10.4172/2155-9821.1000170
Copyright: © 2014 Hewedy OA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Thirteen Rhizobial isolates from root nodules of Faba beans (Vicia faba L.) were obtained from eleven governorates, representing different agro-ecological, agro-climatic and soil conditions in Egypt. A polyphasic approach, including phenotypic and genotypic techniques were used to study their diversity. Rhizobium isolates were examined for their ability to tolerate salt stress and antibiotic resistance. We used four NaCl concentrations ranging from 0.1%, 1%, 3% and 5% in liquid media and plates, moreover we used 24 different antibiotic disks to determine resistance or sensitive among the tested isolates on plates. The obtained results after salt tolerance and antibiotic response of the R. leguminosarium strains showed that the isolate No. RL9 was the superior strain for salt tolerance . The following strains for salt tolerance were the isolates (RL4) and (RL12), although the isolate No. RL11 was the superior strain for antibiotic resistant then isolates No. RL 13 and Rh3. Growth of all isolates were inhibited above 3% NaCl except five isolates RL4, RL9, RL10, RL12 and RL 13. Also the antibiotic resistance of the isolated strains showed a high level of resistance against Cefoxitin (FOX30) and Cefuroxime (Cxm30). The genetic diversity was studied using RAPD-PCR technique and we used specific primer (nodC) amplification. In this study we found that OPE15 and OPG04 primers showed the highest polymorphism level among the tested isolates, however OPJ20 and OPC06 primers showed the lowest polymorphism level.

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