alexa Phenotypic Screening of Aminoglycoside Resistance and t
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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Research Article

Phenotypic Screening of Aminoglycoside Resistance and their Transferability in Clinical Isolates of Klebsiella pneumoniae from India

Nargis Alom Choudhury1, Sunanda Deb1, Anand Prakash Maurya1, Debadatta Dhar Chanda2, Atanu Chakravarty2 and Amitabha Bhattacharjee1*

1Department of Microbiology, Assam University, Silchar, Assam, India

2Department of Microbiology, Silchar Medical College and Hospital, Silchar, Assam, India

*Corresponding Author:
Amitabha Bhattacharjee
Department of Microbiology
Assam University
Silchar, Assam, India
Tel: 918471939088
E-mail: [email protected]

Received: October 20, 2015; Accepted: February 06, 2016; Published: February 11, 2016

Citation: Choudhury NA, Deb S, Maurya AP, Chanda DD, Chakravarty A, et al. (2016) Phenotypic Screening of Aminoglycoside Resistance and their Transferability in Clinical Isolates of Klebsiella pneumoniae from India. J Med Microb Diagn 5:218. doi: 10.4172/2161-0703.1000218

Copyright: © 2016 Choudhury NA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Introduction: Klebsiella pneumonia is an emerging pathogen associated with multidrug resistance both in hospital and community settings. Aminoglycosides, considered to be second line drug for the treatment of such pathogens, become inactive due to acquisition of various resistance determinants by this organism.
Objective: The objective of the study was to screen the aminoglycoside resistant Klebsiella pneumonia from a tertiary referral hospital of northeast India and their transmission dynamics.
Method: A total of 177 consecutive, non-duplicate, clinical isolates of Klebsiella pneumonia were collected from patients from a period of September 2013 to February 2014. Screening for aminoglycoside resistance was performed. Transferability of aminoglycoside resistance was done by transformation assay. Genetic stability was checked by consecutive serial passage of 70 days. Incompatibility types were determined by PCR based replicon typing.
Result: Among 177 clinical isolates, 94 were screened to be resistant towards aminoglycoside group of antibiotics. The aminoglycoside resistance determinant was found to be transferable when transformants were selected in gentamicin (100 μg/ml) screen agar. Coresistance was also shown by these isolates. Gentamicin resistance was lost after 47 consecutive serial passages. F inc type (n = 17) was more predominant, followed by K/B (n = 11), Y (n = 13), I (n = 9) and P (n = 8) when plasmids were typed by PCR based replicon typing.
Conclusion: This study highlighted the transmission dynamics of aminoglycoside resistance determined which pose threat to the treatment option in hospital settings.

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