Phosphoproteomic Analysis of Pancreatic Ductal Adenocarcinoma Cells Reveals Differential Phosphorylation of Cell Adhesion, Cell Junction and Structural Proteins
- *Corresponding Author:
- Dr. Weidong Zhou
Center for Applied Proteomics and Molecular Medicine
George Mason University, 10900 University Blvd, MS 1A9
Manassas, VA 20110, USA
E-mail: [email protected]
Received Date: August 04, 2011; Accepted Date: September 13, 2011; Published Date: September 21, 2011
Citation: Zhou W, Capello M, Fredolini C, Racanicchi L, Piemonti L, et al. (2011) Phosphoproteomic Analysis of Pancreatic Ductal Adenocarcinoma Cells Reveals Differential Phosphorylation of Cell Adhesion, Cell Junction and Structural Proteins. J Proteomics Bioinform 4:170-178. doi:10.4172/jpb.1000186
Copyright: © 2011 Zhou W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this present work, we characterized the phosphoproteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap. We identified more than 700 phosphoproteins from each sample, and revealed differential phosphorylation of many proteins involved in cell adhesion, cell junction, and cytoskeleton. Since post-translational phosphorylation is a common and important mechanism of acute and reversible regulation of protein function in mammalian cells, an understanding of differential phosphorylation of these proteins and resulting signal transduction changes in PDAC will help in comprehending the complex dynamics of tumor invasion and metastasis in pancreatic cancer.