Photoprotective Effects of Blue Light Absorbing Filter against LED Light Exposure on Human Retinal Pigment Epithelial Cells In Vitro
Eva Chamorro#*1, Sergio F Carralero#*1, Cristina Bonnin-Arias1, María Jesús Pérez-Carrasco2, Javier Muñoz de Luna3, Daniel Vázquez Ing3 and Celia Sánchez-Ramos1,2
1Neuro-Computing and Neuro-Robotics Research Group, Universidad Complutense de Madrid, C/ Arcos de Jalón, 118. 28037. Madrid, Spain
2Optometry and Vision Department, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, C/ Arcos de Jalón, 118. 28037. Madrid, Spain
3Optics Department, Facultad de Óptica y Optometría, Universidad Complutense de Madrid. C/ Arcos de Jalón, 118. 28037. Madrid, Spain
#These authors contributed equally to this work
- *Corresponding Author:
- Eva Chamorro
Facultad de Óptica
Optometría y Visión
Universidad Complutense de Madrid. C/ Arcos de Jalón
118. 28037. Madrid, Spain
E-mail: [email protected]
Received date: September 15, 2013; Accepted date: December 12, 2013; Published date: December 18, 2013
Citation: Chamorro E, Carralero SF, Bonnin-Arias C, Pérez-Carrasco MJ, de Luna JM, et al. (2013) Photoprotective Effects of Blue Light Absorbing Filter against LED Light Exposure on Human Retinal Pigment Epithelial Cells In Vitro. J Carcinog Mutagen S6:008. doi: 10.4172/2157-2518.S6-008
Copyright: © 2013 Chamorro E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Over the recent years, several researches have speculated about the effects of Light Emitting
Diodes (LEDs) radiation on retinal epithelium cells (RPE). Worldwide, most people live exposed to LEDs irradiation
incorporated in screens of PCs, phones and TV sets. These lights give rise to the formation of reactive oxygen
species and induce mutagenic mechanisms which lead to apoptosis and consequently to degenerative eye diseases,
such as age-related macular degeneration (AMD). Thus, it is a priority interest to develop appropriate solutions for
the growing industry field of LED light phototoxicity. The aim of this study was to investigate the protective effects of
blue light absorbing filters in order to decrease induced apoptosis on human retinal pigment epithelial cells.
Methods: Human retinal pigment epithelial cells were exposed to 3 light-darkness (12 hours/12 hours) cycles
of white (Tª5400°K), blue (468 nm), green (525 nm) and red (616 nm) LED light. Light irradiance was 5 mW/cm2.
Oxidative stress was evaluated by H2DCFDA staining, mitochondrial membrane potential by TMRM staining, DNA
damage by H2AX histone activation, apoptosis by caspase-3 activation, and cell viability by DAPI.
Results: Our results have shown that the use of a blue light absorbing filter decreased cellular apoptosis by
56-89% and DNA damage by 57-81%. A decrease in ROS level production and an increase in cellular viability was
Conclusion: This study suggests that blue light absorbing filters may protect against LED lighting photo toxicity
and, consequently, provides a photo protector effect.