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Phototoxic Assesment of Benzanthrone and Anthracene by Using NIH-3t3 and L-929 Cell Lines | OMICS International | Abstract
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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Research Article

Phototoxic Assesment of Benzanthrone and Anthracene by Using NIH-3t3 and L-929 Cell Lines

Varun Tobit1*, O. P. Verma1, P. W. Ramteke1 and R. S. Ray2
1Department of Molecular & Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Naini, Allahabad, India
2Indian Institute of Toxicology Research, Lucknow (U.P) India
Corresponding Author : Varun Tobit
Sam Higginbottom Institute of Agriculture
Technology & Sciences, Allahabad, India
E-mail: [email protected]
Received: October 29, 2011; Accepted: December 25, 2011; Published: December 27, 2011
Citation: Tobit V, Verma OP, Ramteke PW, Ray RS (2012) Phototoxic Assesment of Benzanthrone and Anthracene by Using NIH-3t3 and L-929 Cell Lines. J Clin Cell Immunol 3:116. doi:10.4172/2155-9899.1000116
Copyright: © 2012 Tobit V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The Polycyclic Aromatic Hydrocarbons (PAHs) are the most widespread organic environmental pollutants. PAHs are found in motor vehicle exhaust. Some PAHs are found in medicines, dyes, plastics, pesticide, naphthalene balls and wood preservatives. The compounds under the presences of Sun light generally activated and inhibited the cell growth. Hence the present investigation was focused on the maintenances and culturing of NIH-3T3 and L-929 cell lines derived from mouse fibroblast as well as the phototoxicity assessment of PAHs compounds (Anthracene and Benzanthrone) in these cell lines. Monolayer culture of cell lines was grown in DMEM culture medium. Live cells were attached in cryovial check surface and dead cell was mixed in the medium and rise up on the upper layer on the medium. The result showed that the singlet oxygen (1O2) generation by benzanthrone (5-50 ppm) and Anthracene (20-50 ppm) showed the phototoxicity under the exposure of UV-A (5.76 J/cm2), UV-B (2.16 J/cm2) and sunlight 60 min. Rose Bengal (50 ppm) was used as the positive control. Benzanthrone generated the more amount of singlet oxygen as compared to Anthracene while Benzanthrone generated more superoxide as compared to Anthracene.