Phototoxic Assesment of Polycyclic Aromatic Hydrocarbons by Using NIH-3T3 and L-929 Cell Lines
1Department of Molecular Biology & Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture,Technology and Sciences, Naini, Allahabad, Uttar Pradesh, India
- *Corresponding Author:
- Dr. Varun Tobit
Sam Higginbottom Institute of Agriculture
Technology and Sciences, Naini
Allahabad, Uttar Pradesh, India
E-mail: [email protected]
Received Date: April 10, 2011; Accepted Date: May 09, 2011; Published Date: May 13, 2011
Citation: Tobit V, Verma OP, Ramteke PW, Ray RS (2011) Phototoxic Assesment of Polycyclic Aromatic Hydrocarbons by Using NIH-3T3 and L-929 Cell Lines. J AIDS Clinic Res 2:123. doi:10.4172/2155-6113.1000123
Copyright: © 2011 Tobit V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The Polycyclic Aromatic Hydrocarbons (PAHs) are the most widespread organic environmental pollutants. PAHs are found in motor vehicle exhaust. Some PAHs are found in medicines, dyes, plastics, pesticide, naphthalene balls and wood preservatives. The compounds under the presences of Sun light generally activated and inhibited the cell growth. Hence the present investigation was focused on the maintenances and culturing of NIH-3T3 and L-929 cell lines derived from mouse fibroblast as well as the phototoxicity assessment of PAHs compounds (Benzo(a) pyrene, Pyrene) in these cell lines. Monolayer culture of cell lines was grown in DMEM culture medium. Live cells were attached in cryovial check surface and dead cell was mixed in the medium and rise up on the upper layer on the medium. The result showed that the singlet oxygen (1O2) generation by benzo(a)pyrene and pyrene showed the phototoxicity at various concentration from 5-50 ppm under the exposure of UV-A (5.76 J/cm2), UV-B (2.16 J/cm2) and sunlight 60 min. Rose Bengal (50 ppm) was used as the positive control. Pyrene generated the more amount of singlet oxygen as compared to Benzo(a)pyrene while Benzo(a)pyrene generated more superoxide as compared to Pyrene.