Phylogenetic Analysis of Alternaria species Associated with Citrus Black Rot in Iran
Shideh Mojerlou and Naser Safaie*
Department of Plant Pathology, College of Agriculture, Tarbiat Modares University, Tehran, Iran
- *Corresponding Author:
- Naser Safaie
Department of Plant Pathology, Faculty of Agriculture
Tarbiat Modares University
PO Box 14155-336, Tehran, Iran
E-mail: [email protected]
Received date: October 18, 2012; Accepted date: November 17, 2012; Published date: November 22, 2012
Citation:Mojerlou S, Safaie N (2012) Phylogenetic Analysis of Alternaria species Associated with Citrus Black Rot in Iran. J Plant Pathol Microb 3:144. doi:10.4172/2157-7471.1000144
Copyright: ©2012 Mojerlou S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citrus black rot caused by Alternaria spp. is significant post harvest problem in Iran. The causal agent of black rot was originally identified as A. citri by Ellis and Pierce. The genus Alternaria circumscribes considerable morphological diversity and citrus black rot may be caused by more than one morphospecies in addition to A. citri. Morphological identification of small-spored Alternaria spp. is difficult. Phylogenetic analysis was performed on 7 small-spored Alternaria isolates from black rotted citrus fruit in Iran using sequence data from an anonymous locus, OPA10-2. Samples were collected from navel oranges in Mazandaran province in the north of Iran. All isolates caused black rot in a fruit inoculation assay with Navel and Valencia oranges and significant differences were observed between
cultivars and among isolates. No association was found between morphological classification and phylogenetic clade. We identified A. tenuissima (ALT 6) as the causal agents of citrus black rot. Two isolates (ALT8 and ALT9) grouped together, separately from other clades in most parsimonious trees. It suggested that both of them belong to different species. This is the first report of molecular characterization of citrus-associated Alternaria species in Iran and A. tenuissima is reported as the causal agent of citrus black rot in Iran for the first time.