alexa Phylogenetic Analysis of Selected RHDV Strains on the Basis of a Fragment of the Gene Encoding C-Terminal End of VP60 Capsid Structural Protein
ISSN: 2329-9002

Journal of Phylogenetics & Evolutionary Biology
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Research Article

Phylogenetic Analysis of Selected RHDV Strains on the Basis of a Fragment of the Gene Encoding C-Terminal End of VP60 Capsid Structural Protein

Beata Hukowska-Szematowicz1*, Beata Tokarz-Deptuła1, Wiesław Deptuła2
1Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
2Department of Microbiology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
Corresponding Author : Beata Hukowska-Szematowicz
Department of Immunology
Faculty of Biology, University of Szczecin
Felczaka 3c, 71-412 Szczecin, Poland
Tel: +48914441592
Fax: +48914441606
E-mail: [email protected]
Received July 11, 2014; Accepted August 01, 2014; Published August 10, 2014
Citation: Hukowska-Szematowicz B, Tokarz-Deptula, Deptula W (2014) Phylogenetic Analysis of Selected RHDV Strains on the Basis of a Fragment of the Gene Encoding C-Terminal End of VP60 Capsid Structural Protein. J Phylogen Evolution Biol 2:130. doi:10.4172/2329-9002.1000130
Copyright: © 2014 Tokarz-Deptula B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Phylogenetic analyses of RHDV provide information on the affinity of strains and point to evolutionary dependencies among them. The objective of the study was the phylogenetic analysis of six strains of RHD virus, including four Czech strains (CAMPV-351, CAMPV-561, CAMPV-562, CAMPV-558) and two French strains (Fr-1, Fr-2), on the basis of a fragment of the gene encoding C-terminal end of VP60 capsid structural protein. Phylogenetic analysis involved 25 sequences of RHDV homologues obtained from RHDV GenBank. The phylogenetic tree generated for 31 RHDV strains on the basis of a fragment of the gene encoding C-terminal end of VP60 capsid structural protein divided the strains analysed into four genetic groups (G1-G4), whereas the strains analysed were grouped in three genetic groups: G1 (CAMPV-351, CAMPV-562, CAMPV-558), G2 (Fr-1, Fr-2) and G3 (CAMPV-561). The phylogenetic analysis performed for Czech and French strains evidences that the strains feature different evolutionary paths and derive from European strains that caused foci of the plague in Germany and France. The obtained distribution of strains into four genetic groups testifies to their evolution, which is proved by group 4 gathering RHDVa strains.

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