alexa Physiological response to drought and Dehydration respo
ISSN: 2329-8936

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Case Report

Physiological response to drought and Dehydration responsive transcripts (DRTs) from the leaves of water-deficit Indian soybean [Glycine max (L.) Merrill cv NRC7]

Shunmugiah V. Ramesh1*, Subramanian Rajesh1,2, Virender S. Bhatia1and Syed M. Husain1
1ICAR-Directorate of Soybean Research, Indian Council of Agricultural Research, Khandwa Road, Indore, Madhya Pradesh-452001, India
2Centre for Plant Molecular Biology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India
Corresponding Author : Shunmugiah V. Ramesh
Directorate of Soybean Research
Indian Council of Agricultural Research-ICAR
Indore 452001, India
Tel:
+91-9993892302
E-mail:
[email protected]
Received: April 25, 2015 Accepted: May 14, 2015 Published: July 17, 2015
Citation:Ramesh SV, Rajesh S, Bhatia VS, Husain SM (2015) Physiological response to drought and Dehydration responsive transcripts (DRTs) from the leaves of water-deficit Indian soybean [Glycine max (L.) Merrill cv NRC7]. Transcriptomics 3:105. doi:10.4172/2329-8936.1000105
Copyright: ©2015 Ramesh SV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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Abstract

Drought is a major reason for reduced productivity in principal food crops including soybean. Development of drought tolerant varieties is imperative to tide over erratic rainfall conditions in Indian context. Here we studied molecular mechanism governing drought tolerance in Indian soybean cultivar NRC7 to identify drought responsive genic biomarkers. Drought associated physiological parameters like relative water content (RWC) and electrolytic leakage studies under water-deficit condition revealed very low water status in the stressed plants. In addition, membrane damage studies on electrolytic leakages revealed that the stressed plants exhibited greater membrane damage than control plants. Differential display RT-PCR (DD-RT-PCR) identified two drought responsive transcripts (gmDRT1 and gmDRT2) from the soybean cultivar NRC7. The identified transcripts were found to have sequence homology with dehydrin protein and ion ATPase transporters. These biomarkers identified for drought tolerance would also help in engineering drought tolerant soybean cultivars.

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