Phytochemical Analysis and Acute Toxicological Study of Erythrina senegalensis Ethanolic Leaf Extract in Albino Wistar RatsNnama TN*, Asomugha AL, Asomugha RN, Umeasalugo KE and Mgbemena IO
Nnamdi Azikiwe University of Nnewi, Anambra, Nigeria
- *Corresponding Author:
- Nnama TN
Nnamdi Azikiwe University of Nnewi
Tel: +234 808 730 9304
E-mail: [email protected]
Received date: November 22, 2016; Accepted date: December 02, 2016; Published date: December 12, 2016
Citation: Nnama TN, Asomugha AL, Asomugha RN, Umeasalugo KE, Mgbemena IO (2017) Phytochemical Analysis and Acute Toxicological Study of Erythrina senegalensis Ethanolic Leaf Extract in Albino Wistar Rats. Anat Physiol 7:248. doi:10.4172/2161-0940.1000248
Copyright: © 2017 Nnama TN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: Erythrina senegalensis is used to cure wide range of illnesses such as malaria, gastrointestinal disorders, fever, dizziness, asthma. Given its widespread usage, the study sought to investigate the phytochemical analysis and acute oral toxicity (LD50) of E. senegalensis.
Materials and methods: Fresh mature leaf samples of E. senegalensis were collected and phytochemical analysis was carried out appropriately. The acute toxicity study of the leaf extract was determined using modified Lorke’s method. Thirteen adult Wistar rats of both sexes were acclimatized, nine of which were used for the first phase of treatment, and the other four were used for the second phase of acute toxicity testing, while being closely monitored for mortality.
Results: Alkaloids, saponins and flavonoids, were found in moderate quantities; tannins and terpenoids were found in trace amounts, while cardiac glycosides and steroids were not found. The acute oral toxicity of E. senegalensis was greater than 5000 mg/kg of the experimental rats (LD50>5000 mg/kg). Conclusion: E. senegalensis possesses many useful phytoconstituents that contribute to the potency of leaf extract and which made it useful in treatment of many diseases, and that E. senegalensis ethanolic leaf extract has low toxicity in rats, especially when administered orally.