Phytochemical Analysis and Antibacterial and Cytotoxic Properties of Barleria lupulina Lindl. Extracts
Reshma Kumari* and Ramesh Chandra Dubey
Department of Botany and Microbiology, Gurukula Kangri University, Haridwar-249404, India
- *Corresponding Author:
- Reshma Kumari
Department of Botany and Microbiology
Gurukula Kangri University, Haridwar-249404, India
E-mail: [email protected]
Received Date: October 19, 2016; Accepted Date: November 7, 2016; Published Date: November 30, 2016
Citation: Kumari R, Dubey RC (2016) Phytochemical Analysis and Antibacterial and Cytotoxic Properties of Barleria lupulina Lindl. Extracts. J Plant Pathol Microbiol 7:380. doi: 10.4172/2157-7471.1000380
Copyright: © 2016 Kumari R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The ethanolic and aqueous extracts of Barleria lupulina leaves displayed antibacterial activity against five human bacterial pathogens viz., Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi Klebsiella pneumoniae. The ethanolic extract was more inhibitory than the aqueous extract against all the test pathogens, which caused the maximum growth inhibition of P. aeruginosa at 100% concentration. In contrast, aqueous extract did not inhibit the growth of any bacterial pathogens. MIC of ethanolic extract was 2.5 mg/mL against E. coli, S. aureus and P. aeruginosa, and 10.0 mg/mL against S. typhi and K. pneumonia. GC-MS analysis displayed the presence of twelve phytochemical compounds among which benzofuranon, hexadecanoic acid, ethyl 9,12,15-octadecatrienoate, and 3,7,11,15-tetramethyl-2-hexadecanoic acid were the most prominent ones. These extracts also displayed cytopathic effects against HepG2 cell line performed by employing the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and neutral red uptake (NRU) assay which demonstrated the varying levels of cell death of HepG2 cells by ethanolic extract. The ethanolic extract of B. lupulina bears a significant amount of phytochemical compounds that pose antibacterial as well as anti-cancerous properties.