alexa Phytochemical and TLC Profiling of Oroxylum indicum and Milletia pachycarpa
ISSN: 2329-9029

Journal of Plant Biochemistry & Physiology
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Research Article

Phytochemical and TLC Profiling of Oroxylum indicum and Milletia pachycarpa

Lalrinzuali K, Vabeiryureilai M and Ganesh Chandra J*
Department of Zoology, Mizoram University, Aizawl, India
Corresponding Author : Ganesh Chandra J
Department of Zoology
Mizoram University
Aizawl, India
Tel: 91-0389-2330724
Fax: 2330227
E-mail: [email protected]
Received July 04, 2015; Accepted July 22, 2015; Published July 28, 2015
Citation: Lalrinzuali K, Vabeiryureilai M, Ganesh Chandra J (2015) Phytochemical and TLC Profiling of Oroxylum indicum and Milletia pachycarpa. J Plant Biochem Physiol 3:152. doi:10.4172/2329-9029.1000152
Copyright: © 2015 Lalrinzuali K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The humans have used medicinal plants for healthcare since the time immemorial. The systematic phytochemical analysis of traditionally used medicinal plants is needed to establish their use as medicine. Oroxylum indicum and Milletia pachycarpa have been used in India and China to treat various health related disorders. Therefore it was decided to undertake phytochemical and Thin Layer Chromatography (TLC) profiling of different extracts of Oroxylum indicum and Milletia pachycarpa using standard procedures. The dried powder of stem bark of Oroxylum indicum and root bark of Milletia pachycarpa was sequentially extracted in chloroform, ethanol and water. The dried extract of each plant was phytochemically analyzed for the presence of alkaloids, flavonoids, cardiac glycosides, phytosterols, saponins, tannins steroids and phlobatannins. Each extract from both plants was processed for TLC profiling on silica gel using various solvent combinations as mobile phase. The phytochemical analysis showed the presence of alkaloids in chloroform and ethanol extracts, whereas alkaloids were absent in aqueous extract of both Oroxylum indicum and Milletia pachycarpa. The flavonoids were observed in all extracts of both plants. However, cardiac glycosides were absent in the aqueous extract of Milletia pachycarpa. The saponins were detected in all the extracts of both plants except the chloroform extract of Oroxylum indicum. The tannins could not be detected in the aqueous extract of M. pachycarpa. The phlobatannins were absent in all extracts of both plants. Steroids were present in the ethanol extract of both plants. The TLC profiling confirmed the presence of different phytochemicals as evidenced by different Rf values. The present study indicates that the properties of both O. indicum and M. pachycarpa may be due to presence of alkaloids, flavonoids, cardiac glycosides, saponins, tannins and phytosterols.

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