Talha Bin Emran, Mir Muhammad Nasir Uddin, Atiar Rahman, Zia Uddin and Mohaiminul Islam
In this study the ethanol extract, n-hexane extract and chloroform extract of Azadirachta indica were first evaluated for phytochemical study. The phytochemical screening of the three extracts of Azadirachta indica exhibited the presence of important secondary metabolites such as flavonoids, terpenoids, steroids and tannins. The extracts showed potential antimicrobial activities against thirteen different strains of microorganisms. Secondly, they were screened in vitro for cytotoxicity test by brine shrimp lethality bioassay and results illustrated significant (p<0.05) cytotoxicity against Artemia salina. To test the analgesic properties of ethanol extract of Azadirachta indica, hot plate and acetic acid induced writhing methods were used. At two different doses (250 and 500 mg/kg body weight), the analgesic tests were performed on Swiss Albino mice. Also, the anti-inflammatory tests were performed by carrageenan induced paw edema method on long Evans rats at the two different doses of 250 and 500 mg/kg body weight using ethanol extract. Our results indicated that Azadirachta indica possesses remarkable analgesic and anti-inflammatory activity.
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