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ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Phytochemical Characterization of Active Constituents from Extracts of Ixora Javanica D.C Flowers

Sunitha Dontha*, Hemalatha Kamurthy, Bhagavan Raju Mantripragada

Malla Reddy College of Pharmacy, Hyderabad, Telangana, India

*Corresponding Author:
Dontha Sunitha
Associate Professor
Malla Reddy College of Pharmacy
Hyderabad, Telangana-500 014, India
Tel: 9348161224
Fax: 9348161224
E-mail: [email protected]

Received date: August 26, 2015; Accepted date: September 18, 2015; Published date: September 28, 2015

Citation: Kamurthy H, Mantripragada BR, Sunitha D (2015) Phytochemical Characterization of Active Constituents from Extracts of Ixora Javanica D.C Flowers. J Chromatogr Sep Tech 6:294. doi:10.4172/2157-7064.1000294

Copyright: © 2015 Kamurthy H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The investigation of chemical compounds from natural products is fundamentally important for the development of new drugs, especially in view of the vast worldwide flora. The present work is extraction, isolation and characterization of active constituents from Ixora javanica D.C flowers. Freshly collected flowers were shade dried, powdered and subjected to continuous soxhilation with various solvents like petroleum ether (60-80°), ethyl acetate and ethanol (70%) of increasing polarity. All the three obtained extracts were concentrated and after preliminary phytochemical investigation, column chromatographed using silica gel. Three triterpenoidal compounds (Maslinic acid, Ursolic acid and Lupeol), one flavonoidal compound (Formononetin) and two flavonoidal glycoside compounds (Quercetin-3-rutinoside, Quercetin-3-glucoside) have been isolated and further these isolated structures were established by spectral analysis (IR, 1H NMR spectra, 13C NMR and Mass) and direct comparison with authentic samples. This is the first report of occurrence of these compounds from Ixora javanica D.C flowers.

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