Phytochemical Properties and Staining Ability of Red Onion (Allium cepa)Extract on Histological SectionsSunday Ewaoche Itodo*, Steve Oyero, EU Umeh, Amodu Ben and Musa Dauda Etubi
Department of Histopathology and Cytology, Jos University Teaching Hospital, Jos Plataeu State, Nigeria
- *Corresponding Author:
- Sunday Ewaoche Itodo
Department of Histopathology and Cytology
Jos University Teaching Hospital
Jos Plataeu State, Nigeria
E-mail: [email protected]
Received Date: Jun 26, 2014; Accepted Date: August 26, 2014; Published Date: August 28, 2014
Citation: Itodo SE, Oyero S, Umeh EU, Ben A, Etubi MD (2014) Phytochemical Properties and Staining Ability of Red Onion (Allium cepa) Extract on Histological Sections. J Cytol Histol 5:275. doi:10.4172/2157-7099.1000275
Copyright: © 2014 Itodo SE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The staining ability of Allium cepa (red onion) skin extract on tissue sections was determined. The dried red onion skin was ground into small pieces and 300 g (150 g for aqueous and 150 g for ethanolic extraction) of the milled dried red onion skin was used for extraction. Dark red-pigmented granules were obtained. The aqueous and ethanolic extract of mordanted (Potassium aluminum alum and ferric chloride), non-mordanted and acidified solution prepared from the extract were used to stain tissue sections. Cytoplasm of cells and connective tissues were remarkably stained in shades of redish to yellowish brown colours. Preliminary phytochemical screening of these extracts reveals that it contained alkaloid steroid ring, saponin, Tannin, Flavonoid, Cardiac glycoside and anthraquinone. The presence of pigments, saponin, tannin, contributed to the staining ability of the extract.