Phytochemical screening and Antibacterial activity of root extracts of Decalepis hamiltonii Wight & Arn
In the present investigation, preliminary phytochemical and antibacterial activity of petroleum ether, chloroform, ethyl acetate and methanol root extracts of Decalepis hamiltonii were examined. The phytochemical analysis revealed the presence of active ingredients such as glycosides, steroids, flavonoids, phenols, terpenoids, saponins and tannins in the root extracts of Decalepis hamiltonii. The antibacterial activity was performed by disc diffusion method using different concentrations of solvent extracts. Seven bacterial pathogen such as gram positive - Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and gram negative-Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi were used as test organisms. Among the four solvents used methanol extract was found to bemore active against most of the tested pathogenic bacteria at higher concentrations, as they showed potential phytochemical constituents. Among all the tested organisms Klebsiella pneumoniae was found to be more resistant by all the extracts, where as Escherichia coli, Pseudomonas aeruginosa, and salmonella typhi showed moderate resistant and Staphylococcus aureus, Bacillus cereus and Bacillus subtilis showed least resistant against all the extracts. The results of present study supports traditional usage Decalepis hamiltonii and also suggest that methanol root extract possess compounds with antimicrobial property that can be used as antimicrobial agents in new drug for the therapy of infectious diseases caused by pathogens.