Phytoestrogenic Compounds and Their Synthetic Analogs, Contrary to Estradiol- 17b Stimulates Human Derived Female Cultured Bone Cells in Hyperglycemic Conditions
- *Corresponding Author:
- Dr. Dalia Somjen PhD
Institute of Endocrinology
Metabolism and Hypertension
Tel-Aviv Sourasky Medical Center
6 Weizman St. Tel-Aviv, 64239 Israel
Tel: 972 3 6973812
Fax: 972 3 6973421
E-mail: [email protected] tasmc.health.gov.il
Received date: January 06, 2011; Accepted date: March 07, 2011; Published date: March 12, 2011
Citation: Somjen D, Katzburg S, Tamir S, Sharon O, Hendel D, et al. (2011) Phytoestrogenic Compounds and their Synthetic Analogs, Contrary to Estradiol- 17b Stimulates Human Derived Female Cultured Bone Cells in Hyperglycemic Conditions. J Steroids Hormon Sci 2:105. doi: 10.4172/2157-7536.1000105
Copyright: © 2011 Somjen D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cultured female- derived human osteoblasts (hObs) responded by different parameters to the phytoestrogens: daidzein (D), glabrene (Gla) and glabridin (Glb), to their synthetic derivatives; carboxy-daidzein (cD) and to estradiol- 17? (E2). Since the skeletal protective effects of estrogens are not discernible in diabetic women, we tested the effects of these compounds on hObs grown in growth medium with high glucose (HG; 9.0g/L; 44 mM) compared to normal glucose (NG; 4.5g/L; 22 mM) using the stimulation of creatine kinase specific activity (CK) and 3[H] thymidine incorporation in to DNA (DNA) as hormonal responsiveness markers. HG slightly increased DNA and CK in hObs. Stimulations by E2 was abolished and by cD and D was slightly decreased in HG, but not by Gla and Glb in both age groups. Growing hObs in HG upregulated the expression of mRNA of both ER? and ER? in cells from pre- but not from post-menopausal women. Cells from both age groups express also mRNA for 25 hydroxy vitamin D3 1-? hydroxylase and showed enzymic activity which were down-regulated by HG in both age groups. Whether Gla and Glb act differentially via ERs and/or 1-? hydroxylase is not yet established.Since these compounds are active even in HG, they might be used for treating hyperglycemic/diabetic women.