alexa Identification and Biotyping of <em>Escherichia coli</em> from Diarrheic Lambs in and Around Debre Birhan Town, Ethiopia | OMICS International| Abstract

ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology

  • Research Article   
  • J Environ Anal Toxicol 2013, Vol 3(6): 188
  • DOI: 10.4172/2161-0525.1000188

Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debre Birhan Town, Ethiopia

Metasebia Aklilu1*, Tesfaye Sisay2, Genene Tefera3 and Belay Tekalign4
1College of Veterinary Medicine and Agriculture, Addis Ababa University, Debrezeite, Ethiopia
2Department of Health Biotechnology, Institute Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia
3Institute of Biodiversity center, , Addis Ababa, Ethiopia
4Mereced University, , California, USA
*Corresponding Author : Metasebia Aklilu, Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, Addis Ababa, Ethiopia, Email: [email protected]

Received Date: Jul 25, 2013 / Accepted Date: Aug 29, 2013 / Published Date: Sep 02, 2013

Abstract

Background: Infectious diarrhea is the most significant cause of morbidity and mortality in neonatal dairy sheep throughout the world. This is the first study conducted on isolation and biotyping of E. coli from lamb diarrhea in and around Debre Birhan. The present study was undertaken with the objective of isolation, omnilog characterization and biotyping of E. coli isolates from faecal samples around Debrebrhan.
Methods: A cross-sectional study was conducted from October, 2012 to April, 2013. The study focused on lambs less than three months of age showing clinical symptoms of diarrhea. Standard cultural and Omnilog tests were done to identify E. coli species and biotypes; descriptive statistics and Chi-square were used to analyze the collected data.
Results: From a total of 100 diarrheic lambs examined, 84% were found to be positive for E. coli. On the basis of fermentation reactions of sugars viz. dulcitol, raffinose, rhamnose, salicin, starch and sucrose, 70 isolates utilized one or more sugar while 14 isolates failed to utilize any of the sugars. 70 E. coli isolates were biotyped into 15 different combinations. Association between different age groups and occurrence of lamb diarrhea caused by E. coli strains showed a significant association (p < 0.05). From the questionnaire findings, 60% of all the health problems in lambs were due to diarrhea where young sheep were more affected.
Conclusion: pathogenic E. coli has the potential to cause sheep diarrhea. The distribution of the E. coli isolates into different biotypes indicates the diverse nature of the organism. Therefore, further detailed study should be carried out to understand the role of E. coli in lamb diarrhea and identify the virulent strains involved.

Keywords: Biotyping; Debre Birhan; Lamb diarrhea; Omnilog; Sugars; Ethiopia

Citation: Aklilu M, Sisay T, Tefera G, Tekalign B (2013) Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debre Birhan Town, Ethiopia. J Environ Anal Toxicol 3: 188. Doi: 10.4172/2161-0525.1000188

Copyright: © 2013 Aklilu M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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