alexa Inhibitory Effect of Alpha-Crystallins on VEGF-Induced Proliferation of Retinal Microvascular Endothelial Cells | OMICS International| Abstract

ISSN: 2155-9570

Journal of Clinical & Experimental Ophthalmology

  • Research Article   
  • J Clin Exp Ophthalmol 2018, Vol 9(1): 715
  • DOI: 10.4172/2155-9570.1000715

Inhibitory Effect of Alpha-Crystallins on VEGF-Induced Proliferation of Retinal Microvascular Endothelial Cells

Shogo Arimura1, Yoshihiro Takamura1*, Seiji Miyake1, Atsushi Kojima2, Shuji Sakurai2, Kazuhiro Tsuruma2, Hideaki Hara2 and Masaru1
1Department of Ophthalmology, Faculty of Medical Science, University of Fukui, 23-3, Shimoaizuki, Eiheiji, Yoshida, Fukui, Japan
2Department of Biofunctional Evaluation, Faculty of Molecular Pharmacology, Gifu Pharmaceutical University, Daigakunishi, Gifu, Japan
*Corresponding Author : Yoshihiro Takamura, Department of Ophthalmology, Faculty of Medical Science, University of Fukui, 23-3, Shimoaizuki, Eiheiji, Yoshida, Fukui, Japan, Tel: +81776618403, Fax: +817768131, Email: [email protected]

Received Date: Feb 14, 2018 / Accepted Date: Feb 22, 2018 / Published Date: Feb 28, 2018

Abstract

Purpose: Lens extraction surgery with ischemic retinopathy may trigger neovascularization, suggesting that lens removal alters the ocular concentrations of angiogenesis regulatory factors. This study aims to identify angiogenic or anti-angiogenic factors exhibiting altered expression in the vitreous after lens extraction in rabbits.
Methods: Vitreous samples after lens extraction were analyzed by two dimensional fluorescence difference gel electrophoresis (2D-DIGE) followed by mass spectrometry to identify candidate factors indicating expression changes. Western blotting and real-time polymerase chain reaction (PCR) were performed to semi-quantify protein and gene expression changes. The identified factors were tested by assessing effects on vascular endothelial growth factor (VEGF)-induced proliferation of human retinal microvascular endothelial cells (HRMECs).
Results: Three spots with reduced expression were identified as αA-crystallin by 2D-DIGE and mass spectrometry. Western blotting revealed that the protein levels in vitreous of αA- and αB-crystallin were significantly lower after lens extraction. αB-crystallin suppressed the proliferation of VEGF-stimulated HRMECs, and the combination of αA- and αB-crystallin was more effective than αB-crystallin alone.
Conclusion: The depletion of vitreal α-crystallins after lens extraction may increase VEGF-induced angiogenesis, thereby contributing to adverse outcomes such as vitreous hemorrhage, proliferative membrane, and vision loss after cataract surgery for ischemic retinopathy patients.

Keywords: Lens extraction surgery; Angiogenesis regulatory factor; Cataract; Ischemic retinopathy; Cell proliferation assay

Citation: Arimura S, Takamura Y, Miyake S, Kojima A, Sakurai S, et al. (2018) Inhibitory Effect of Alpha-Crystallins on VEGF-Induced Proliferation of Retinal Microvascular Endothelial Cells. J Clin Exp Ophthalmol 9: 715. Doi: 10.4172/2155-9570.1000715

Copyright: © 2018 Arimura S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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