alexa Pioglitazone HCl Levels and Its Pharmacokinetic Application in Presence of Sucralose in Animals Serum by HPLC Method
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

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Research Article

Pioglitazone HCl Levels and Its Pharmacokinetic Application in Presence of Sucralose in Animals Serum by HPLC Method

Lina Tamimi1, Wael Abu Dayyih1*, Nidal Qinna2, Eyad Mallah1 and Tawfiq Arafat1

1Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences-University of Petra, Jordan

2Department of Pharmacology and Biomedical Sciences-University of Petra, Jordan

*Corresponding Author:
Wael Abu Dayyih
Associate Professor
Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy
Faculty of Pharmacy and Medical Sciences- University of Petra
Amman, Jordan
Tel: 0096265799555-8119
E-mail: wabudayyih@uop.edu.jo

Received date: September 30, 2014; Accepted date: November 14, 2014; Published date: November 20, 2014

Citation: Tamimi L, Abu Dayyih W, Qinna N, Mallah E, Arafat T (2014) Pioglitazone HCl Levels and Its Pharmacokinetic Application in Presence of Sucralose in Animals Serum by HPLC Method. Pharm Anal Acta 5:318. doi:10.4172/2153-2435.1000318

Copyright: © 2014 Tamimi L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Purpose: to develop a simple, valid and rapid chromatographic method for quantifying pioglitazone HCl in rats serum in order to study the pharmacokinetic parameters of pioglitazone HCl in rats serum fed with sucralose simultaneously for examination of interaction possibility between pioglitazone HCl and sucralose in rats. Methods: In our developed method of analysis, mobile phase was consisted of [51.50%] acetonitrile and [48.50%] 0.025 mM ammonium acetate with pH of 8, column of separation was C8 at temperature of 40°C using injection volume of 90 μl, mobile phase flow rate was 1 ml/min and samples run time was 10 min, the signals were monitored and analyzed at λ=269 nm and sildenafil citrate was used as internal standard. Pioglitazone was given to rats orally of [10mg/kg] dose while sucralose was given with [11 mg/kg/day] dose. Results: A successful HPLC method was validated and developed to quantify pioglitazone HCl in rats serum, overall intra-day precision and accuracy were reasonable with CV % values range [0.16-3.54] and accuracy % range [ 98.4-107.9], while inter-day precision and accuracy showed accepted precision with CV% range [ 0.15- 4.13] and accuracy % range [99.35-103.99]. The coefficient of correlation was 0.9991 with reasonable sensitivity and selectivity. Combination effect of pioglitazone with sucralose on pioglitazone serum profile was demonstrated as strong statistical effect according to Cohen’s d and significant P values too. Conclusion: A successful HPLC method was validated and developed to quantify pioglitazone HCl in rats serum, combination effect of pioglitazone with sucralose over all time intervals of pioglitazone serum profile was demonstrated as strong statistical effect.

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