alexa Plasma Protein Profiling of Breast Cancer Patients of North Indian Population: A Potential Approach to Early Detection
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Plasma Protein Profiling of Breast Cancer Patients of North Indian Population: A Potential Approach to Early Detection

1Department of Computer Science, San Diego State University, San Diego, 92182 CA, USA Marko Vuskovic1*, 2Department of Cardiothoracic Surgery, NYU School of Medicine, New York, 10016 NY, USA Anna-Maria Barbuti2, 2Department of Cardiothoracic Surgery, NYU School of Medicine, New York, 10016 NY, USA Emma Goldsmith-Rooney2, 2Department of Cardiothoracic Surgery, NYU School of Medicine, New York, 10016 NY, USA Laura Glassman2, 3Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Nicolai Bovin3, 2Department of Cardiothoracic Surgery, NYU School of Medicine, New York, 10016 NY, USA Harvey Pass2,
5Department of Environmental Medicine, NYU School of Medicine, New York, 10016 NY, USA Kam-Meng Tchou-Wong5, 4Lanzhou University School of Public Health, Lanzhou, Gansu 730000, China Meichi Chen4, 4Lanzhou University School of Public Health, Lanzhou, Gansu 730000, China Bing Yan4, 4Lanzhou University School of Public Health, Lanzhou, Gansu 730000, China Jingping Niu4, 5Department of Environmental Medicine, NYU School of Medicine, New York, 10016 NY, USA Qingshan Qu5, 5Department of Environmental Medicine, NYU School of Medicine, New York, 10016 NY, USA Max Costa5 and 2Department of Cardiothoracic Surgery, NYU School of Medicine, New York, 10016 NY, USA Margaret Huflejt2
 
Corresponding Author : Marko Vuskovic
Department of Computer Science
San Diego State University
San Diego 92182 CA, USA
Tel: 858-344-7857
E-mail: [email protected]
 
Received November 01, 2013; Accepted December 27, 2013; Published December 30, 2013
 
Citation: Vuskovic M, Barbuti AM, Goldsmith-Rooney E, Glassman L, Bovin N, et al. (2013) Plasma Anti-Glycan Antibody Profiles Associated with Nickel level in Urine. J Proteomics Bioinform 6:302-312. doi:
 
Copyright: © 2013 Vuskovic M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
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Abstract

Study background: Over the last two decades worldwide the mortality due to breast cancer (BC) raises catastrophically. Unfortunately, clinicians are still seeking for reliable resources for early speculations. Recently, the approach of investigative plasma based molecular markers builds a new hope for the early stage diseases.

Methods: Blood samples of healthy and BC individuals from North Indian women (n=25) were collected. In order to increase the detecting sensitivity of protein of interest, the pooled plasma samples were subjected to Multiple affinity removal system (MARS) Hu-14 and PROTEOPREP 20 and further processed by 2D-PAGE/LCMS/ MS analysis. Finally, the levels of selected proteins were independently estimated by western blotting and immunohistochemistry.

Results: The comparative 1D-SDS page and 2DE gel analysis showed the high proficiency of Hu-14 MARS for diminution of HAPs. The proteome profile of pooled plasma sample yielded total 24 differentially expressed spots in BC over control. Among them, the identified 3 up-regulated proteins, Serpin peptidase inhibitor clade A (privilege 92%; 95%CI 79.36-104.63), Apolipoprotein A IV (privilege 72%; 95%CI 52.4-91.6), Apolipoprotein AI (privilege 92%; 95%CI 79.36-104.63), and 1 down-regulated α-2-HS glycoprotein (privilege 76%; 95%CI 57.26-94.74) spots were of high significance (p<0.05). These proteins are involved in pathogenesis of cancer and play an important role in the regulation of metastasis. However their discerned molecular mechanisms evaluation related to BC is ongoing to scrutinize its clinical utility. Importantly, the results of immunochemical quantifications were found consistent with the changes in 2-DE check for all candidates apart from α-2-HS glycoprotein, where the immunohistochemistry analysis was not correlated well with 2DE and immunoblotting.

Conclusion: This preliminary work presents a panel of differential plasma proteins in BC patients, validated by combining routine clinical immunochemical reactions for the first time in North India. Further, in order to establish a cause and effect relationship of these highly modulated proteins with BC needs large scale sample size investigation.

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