alexa Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection | OMICS International | Abstract
ISSN: 2327-5073

Clinical Microbiology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection

Sabrina Suhani, Auditi Purkaystha, Musammat Kulsuma Begum, Md. Jahidul Islam and Abul Kalam Azad*

Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

*Corresponding Author:
Abul Kalam Azad
Shahjalal University of Science and Technology, Sylhet, Bangladesh
Tel: +880 1715 160921
E-mail: [email protected]

Received date: June 07, 2017; Accepted date: June 30, 2017; Published date: July 05, 2017

Citation: Suhani S, Purkaystha A, Begum MK, Islam MJ, Azad AK (2017) Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection. Clin Microbiol 6:284. doi: 10.4172/2327-5073.1000284

Copyright: © 2017 Suhani S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Background: Escherichia coli causes urinary tract infection (UTI), one of the most frequent bacterial infections in humans, shows resistance to various commonly prescribed antibiotics. This is termed as multi drug resistance, which is increasing among E. coli. The multi-drug resistant E. coli isolates harbor multiple plasmids that might be responsible for antibiotic resistance. The resistance can be transferred among bacteria through horizontal transfer of the genetic materials.

Objectives: We undertook this study 1) to find a link between antibiotic resistance and plasmid of E. coli from UTI patients of Sylhet region of Bangladesh 2) to analyze the spread of the plasmid mediated antibiotic resistant gene between E. coli isolates.

Methods: Conjugation experiment was carried out in Luria Broth with E. coli DH5α as recipient. For transformation, competent cells were prepared using E. coli DH5α. Plasmid isolation was done by mini alkaline lysis method and plasmid was extracted from agarose gel and transferred to the competent cell.

Results: After conjugation, the donor E. coli isolate that showed resistance to amoxicillin (AMX), ciprofloxacin (CIP) and ceftriaxone (CTR) tranferred its AMX and CIP resistance to the recipient E. coli DH5α, that was previously sensitive to all antibiotics. After transformation, the recipient E. coli DH5α became resistant to CIP and AMX, while the donor showed resistance to gentamycin (CN), ceftriaxone (CTR), amoxicillin (AMX), ciprofloxacin (CIP), cefixime CFM, and cotrimoxazole (COT). Plasmid extracted from the transformant revealed that, three genes (3 kb, 5 kb and 20 kb) have been transferred from the donor to recipient.

Conclusion: Plasmids responsible for amoxicillin and ciprofloxacin were transferred


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7