Polyester Fleeces used as an Artificial Interstitium Influence the Spatial Growth of Regenerating Tubules
Anne Glashauser, Lucia Denk and Will W. Minuth*
Molecular and Cellular Anatomy, University of Regensburg, University Street 31, D-93053 Regensburg, Germany
- Corresponding Author:
- Dr. Will W. Minuth
Molecular and Cellular Anatomy, University of Regensburg
University Street 31, D-93053 Regensburg, Germany
Tel.:+49 (0)941 943 2876
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Email: [email protected]
Received date: July 19, 2011; Accepted date: July 27, 2011; Published date: July 29, 2011
Citation: Glashauser A, Denk L, Minuth WW (2011) Polyester Fleeces used as an Artificial Interstitium Influence the Spatial Growth of Regenerating Tubules. J Tissue Sci Eng 2:105. doi:10.4172/2157-7552.1000105
Copyright: © 2011 Glashauser A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In regenerative medicine the use of stem/progenitor cells is a valuable therapeutical option for the regeneration of diseased tissues and organs. However, the secure application of cell-based therapies for the treatment of renal failure requires exact information regarding the mechanisms of parenchyma development in combination with different kinds of biomaterials. Recently, we demonstrated that application of stem/progenitor cells in combination with a polyester fleece I-7 as an artificial interstitium supported the spatial generation of tubules during perfusion culture in chemically defined Iscove`s Modified Dulbecco’s Medium containing aldosterone (1x10-7M). In the present experiments we investigated if the use of different polyester fleeces (Posi-4 and Posi-5 in comparison to I-7) has any effect on generation, differentiation and spatial development of tubules. In consequence, immunohistochemistry, transmission electron microscopy and scanning electron microscopy were performed. Since the specimens were not coated by extracellular matrix proteins, unique insights in the contact zone between the basal lamina, interstitial cells and surrounding polyester fibers can be obtained. Analyzing the specimens developing in I-7, Posi-4 and Posi-5 polyester fleeces by means of immune histochemistry and transmission electron microscopy illuminated that no cell biological differences could be observed. In contrast, scanning electron microscopy of generated tubules demonstrated that a difference in spatial distribution and different diameters of tubules could be registered. It is concluded that tested I-7, Posi-4 and Posi-5 polyester fleeces appear as promising candidates to shelter stem/progenitor cells after implantation although they exhibit different spatial growth pattern.