alexa Potential Clinical Usefulness of the Polymerase Chain R
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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Research Article

Potential Clinical Usefulness of the Polymerase Chain Reaction Test to Detect Pathogens Causing Sepsis

Asako Matsushima1*, Osamu Tasaki2, Takeshi Shimazu3, Seishi Asari4, Keigo Kimura4, Tomomi Sakata4, Hisashi Sugimoto5

1Department of Trauma, Critical Care Medicine and Burn Center, Social Insurance Chukyo Hospital, Nagoya Japan

2Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Japan

3Department of Emergency Room Medicine, Kinki University, School of Medicine, Japan

4Laboratory for Clinical Investigation, Osaka University Graduate School of Medicine, Japan

5Hoshigaoka Koseinenkin Hospital, Japan

*Corresponding Author:
Asako Matsushima
Department of Trauma,
Critical Care Medicine and Burn Center, Japan,
Tel: 81-52-691-7151
Fax: 81-52-692- 5220
E-mail: [email protected]

Received Date: January 19, 2012; Accepted Date: March 01, 2012; Published Date:March 09, 2012

Citation: Matsushima A, Tasaki O, Shimazu T, Asari S, Kimura K et al. (2012) Potential Clinical Usefulness of the Polymerase Chain Reaction Test to Detect Pathogens Causing Sepsis. J Medical Microbiol Diagnosis 1:106. doi: 10.4172/2161-0703.1000106

Copyright: © 2012 Matsushima A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Objective: A real-time polymerase chain reaction (PCR) test is expected for early and precise detection of pathogens in blood. In this study, we compared the ability of the PCR test and blood culture to detect pathogens in the blood of patients with sepsis.

Methods: Patients who were diagnosed as or suspected of having sepsis were included in this prospective observational study. A whole blood sample for PCR test was obtained serially simultaneously with the blood culture sample, and the results were compared.

Results: We obtained 93 samples from 26 patients; 69 samples were obtained during the septic condition, and 24 samples were from the non-septic condition. Origins of sepsis were pneumonia in 9 patients, necrotizing fasciitis in 5 patients, and other causes in 12 patients. In the septic condition, rates of positive results were 29.0% for the PCR test and 23.2% for blood culture. Sample contamination occurred in 1 PCR test sample and 5 blood culture samples. In positive PCR samples, 16 of 20 samples were obtained during sepsis after beginning administration of broad- spectrum antibiotics, whereas 5 of 12 samples, except for contaminated samples, were positive in blood culture.

Conclusion: In sepsis, the PCR test detected more bacteria than did blood culture even after administration of empirical antibiotics, which might contribute to precise diagnosis of the bacteremic cause of sepsis

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