Potential of Indigenous Enzymatic Activities, Nitric Oxide and Ceruloplasmin in Bovine Milk to Diagnose Subclinical Mastitis
U.K De* and Reena Mukherjee
Division of Medicine, Indian Veterinary Research Institute, Izatnagar-243122 (UP), India
- *Corresponding Author:
- Dr. U K De
Scientist, Division of Medicine
Indian Veterinary Research Institute
Izatnagar-243122 (UP), India
Tel : +91-581-230587
Fax: +91 581 2301940
E-mail: [email protected]
Received date: May 12, 2011; Accepted date: August 25, 2011; Published date: October 30, 2011
Citation: De UK, Mukherjee R (2011) Potential of Indigenous Enzymatic Activities, Nitric Oxide and Ceruloplasmin in Bovine Milk to Diagnose Subclinical Mastitis. J Veterinar Sci Technol 2:109. doi:10.4172/2157-7579.1000109
Copyright: © 2011 De UK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The objective of this present study was to evaluate some indigenous enzymatic activities, nitric oxide production in milk cells and ceruloplasmin (C p ) level in milk as diagnostic indicators of bovine subclinical mastitis (SCM). The crossbred cows were screened with California mastitis test (CMT) to determine the status of SCM in mammary glands. Based on CMT point score and somatic cell count (SCC) of milk, the mammary quarters were classified as healthy (negative CMT reaction and SCC< 3 lakhs/ ml) and SCM (CMT reaction + or more and SCC>10 lakhs/ ml). The results of the study revealed that SCC, cyclooxygenase (COX), myeloperoxidase (MPO), lactoperoxidase (LP), nitric oxide (NO) and ceruloplasmin (C p ) levels were significantly (p<0.05) higher in milk collected from SCM quarters compared to healthy quarters. Pearson’s correlation (r) of paired data of individual SCM infected quarters revealed the existence of a significant positive (P < 0.01) correlation among SCC and COX activity, LP activity, MPO activity and NO production. However, no significant correlation between SCC and Cp level was observed in milk samples of SCM infected quarters. Therefore, alteration of these enzyme activities, NO production and C p activity in milk could be used as an alternative diagnostic tool to screen for SCM.