Potential Spread of Methicillin-Resistant Staphylococcus aureus Recovered from Patients with Bloodstream InfectionRenata MF Gomes1, Maria Rosa Q Bomfim3, Mariana JV Trindade1, Luiz M Farias1, Maria Auxiliadora R Carvalho1, José Carlos Serufo2, Simone G Santos*1
1Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Pampulha, CEP. 31.270-901, Belo Horizonte, Minas Gerais, Brasil
- *Corresponding Author:
- Simone Gonçalves dos Santos
Departamento de Microbiologia
Instituto de Ciências Biológicas
Universidade Federal de Minas Gerais (UFMG)
Avenida Antônio Carlos, 6627
Pampulha, CEP.31.270-901, Belo Horizonte
Minas Gerais, Brasil
E-mail: [email protected]
Tel: 55 31 34092761
Fax: 55 31 34092730.
Received date: March 9, 2015; Accepted date: May 13, 2015; Published date: May 18, 2015
Citation: Renata MF Gomes, Maria Rosa Q Bomfim, Mariana JV Trindade, Luiz M Farias, Simone G Santos, et.al (2015) Potential Spread of Methicillin-Resistant Staphylococcus aureus Recovered from Patients with Bloodstream Infection. Chemo Open Access 4:149. doi:10.4172/2167-7700.1000149
Copyright: ©2015 Gomesa R,This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bloodstream infection (BSI) caused by methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide public health problem, and is associated with high morbidity and mortality. Our aim was to evaluate antimicrobial resistant genes, to characterize the Staphylococcal cassette chromosome elements (SCCmec) and the genetic diversity of MRSA strains recovered from the BSI of five Hospitals in Belo Horizonte, Brazil. Fifty-six MRSA isolates were identified by the Vitek II system, and by the agar dilution method to determine the minimum inhibitory concentration. Polymerase chain reaction (PCR) was performed to detect coagulase (coa), methicillin (mecA) aminoglycosides (aaca-aphD), macrolides, lincosamides (ermA/ermB/ermC) and beta-lactams (blaz) genes, as well as chromosomal SCCmec type. The genetic diversity was carried out by ribotyping and intergenic repetitive sequences ERIC/PCR
analysis. The mecA gene was detected in 84% of strains. At least one of the genes was present in the isolates from hospitals studied; the more frequent combinations were ermA/mecA and ermA/ermB/ermC (78.6% of samples). The SCC Studies have shown that such bacteria may be carriers of the ermA, ermB and ermC genes, type III was the most prevalent, followed by subtype IIIa. Ribotyping and ERIC-PCR results showed a variety of MRSA strains and suggest that certain clonal populations are circulating among the hospitals studied for different routes that should 16 be better investigated.