alexa Pre-challenge Evaluation of Immune Response in Serum Cytokines (Th1 and Th2) and T-cell Markers (CD4, CD8, CD3, and CD25) Following Administration of New Formulated Leishmania Vaccine in Balb/c Mice| Abstract
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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  • Research Article   
  • J Clin Cell Immunol 2017, Vol 8(6): 533
  • DOI: 10.4172/2155-9899.1000533

Pre-challenge Evaluation of Immune Response in Serum Cytokines (Th1 and Th2) and T-cell Markers (CD4, CD8, CD3, and CD25) Following Administration of New Formulated Leishmania Vaccine in Balb/c Mice

Afshineh Latifynia1,2*, Mohamad Hosein Niknam1, Samad Farashi Bonab1, Bita Ansaripour1, Zahra Gheflati1 and Mohammad Javad Gharagozlou3
1Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
2Research Center for Immunodeficiencies, Childeren’s Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
3Department of Pathology, School of Veterinary Medicine, University of Tehran, Tehran, Islamic Republic of Iran
*Corresponding Author : Afshineh Latifynia, Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran, Tel: 982166432620, Email: [email protected]

Received Date: Aug 09, 2017 / Accepted Date: Nov 30, 2017 / Published Date: Dec 07, 2017

Abstract

Leishmaniasis is considered an endemic disease that is a major public health concern in Iran and elsewhere. It is suggested that an effective immune response against leishmaniasis is T cells-mediated immunity that provides immunity against leishmania infection. The formulation and production of an immunogenic, effective, and safe vaccine to control leishmania infection is a necessity. Due to the complexity of the biological behavior of the leishmania parasite and its host immune response, the formulation and production of a safe and a protective vaccine is a difficult but worthwhile endeavor to tackle health problems.

Methods: In this study, we evaluated pre-challenged immune responses related to the Th1 (IFN-gamma and IL-12) and Th2 (IL-4 and IL-10) cytokine profiles, and the CD4, CD8, CD3, and CD25 markers of T cells. This measurement was followed by vaccination accompanied by two one-week interval boosters with the leishmania major antigen preparations adjuvant with BCG or alcoholic extract of Teucrium polium plant or both at 100 and 200 micrograms of the crude antigen/0.1 ml per mouse. This experiment was performed on six groups of leishmania-susceptible Balb/c mice.

Results: The statistical analysis of the data related to the T cells or lymphoid cells with the different markers, including CD8, CD3, and CD25, indicated that there were no significant differences between seven groups of animals; however, the differences were significant when the CD4 T cells were considered. On comparing the cytokines levels in the antigen-injected groups and the control group, the results showed only significant differences in serum IL-12 levels. Conclusion: It was concluded that, as shown in previous studies and the present research, the vaccine could not only induce a protective immunity in Balb/c mice, but it also did not produce deleterious responses as shown through clinical monitoring and even resulted in a 100% survival rate of the experimental animals.

Keywords: New vaccine; Th1 and Th2 cytokines and CDs; pre-challenge; Balb/c

Citation: Latifynia A, Niknam MH, Bonab SF, Ansaripour B, Gheflati Z, et al. (2017) Pre-challenge Evaluation of Immune Response in Serum Cytokines (Th1 and Th2) and T-cell Markers (CD4, CD8, CD3, and CD25) Following Administration of New Formulated Leishmania Vaccine in Balb/c Mice. J Clin Cell Immunol 8:533. Doi: 10.4172/2155-9899.1000533

Copyright: © 2017 Latifynia A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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