alexa Preliminary Development of a Magnetically Assisted Test
ISSN: 2475-7586

Journal of Biomedical Engineering and Medical Devices
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Preliminary Development of a Magnetically Assisted Test Strip (MATS) Cartridge and Fluorescent DNA Aptamer-Magnetic Bead Quantum Dot Sandwich Assays for Multiplexed Food Safety Applications

John G Bruno* and Taylor Phillips
Pronucleotein Biotechnologies, Inc. San Antonio, USA
Corresponding Author : John G. Bruno
Pronucleotein Biotechnologies
Inc 4100 NW Loop 410, Suite 230
San Antonio TX 78229 USA
Tel: 210-731-0015
E-mail: [email protected]
Received: December 11, 2015 Accepted: December 28, 2015 Published: December 31, 2015
Citation: John GB, Taylor P (2016) Preliminary Development of a Magnetically Assisted Test Strip (MATS) Cartridge and Fluorescent DNA Aptamer-Magnetic Bead Quantum Dot Sandwich Assays for Multiplexed Food Safety Applications. J Biomed Eng Med Devic 1:103.
Copyright: © 2016 John G. Bruno, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at Pubmed, Scholar Google
 

Abstract

Preliminary development of a simple mesofluidic multi-channel plastic cartridge with underlying external magnet to drag DNA aptamer-coated paramagnetic beads through fluids in the channels while developing a sandwich assay with quantum dot-conjugated reporter aptamers is described. This approach is superior to traditional lateral flow test strips in several ways including: 1) the ability to control the speed of lateral flow in the channel versus conventional nitrocellulose analytical membranes with fixed wicking times. 2) The use of aptamers for potentially greater affinity and consistency from batch-to-batch versus comparable antibodies. 3) Superior fluorescence efficiency and intensity provided by quantum dots versus conventional fluorescent dyes and 4) the ability to multiplex based on the various colored emissions of different sized quantum dots when excited with a single ultraviolet source. Development of the system from concept to prototype is described along with illustration of sensitive system performance for several food safety-related targets. The system is also clearly adaptable to rapid multiplex detection and sensitive quantitation of clinical biomarkers, drugs, environmental, veterinary or other target analytes.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords