Premnine HCl Estimation in Selected Formulations of Ã¢ÂÂDashmulÃ¢ÂÂ and in Chloroform Extract of Premna integrifolia L. by a Selective, Validated and Developed HPTLC Fingerprint Method
- *Corresponding Author:
- Attarde DL
Department of Pharmacognosy
Mahatma Gandhi Vidyamandir’s Pharmacy College
Panchavati, Nashik, Maharashtra, India
E-mail: [email protected]
Received Date: February 05, 2017; Accepted Date: February 14, 2017; Published Date: February 20, 2017
Citation: Attarde DL, Pal SC, Bhambar RS (2017) Premnine HCl Estimation in Selected Formulations of ‘Dashmul’ and in Chloroform Extract of Premna integrifolia L. by a Selective, Validated and Developed HPTLC Fingerprint Method. J Chromatogr Sep Tech 8:352. doi: 10.4172/2157-7064.1000352
Copyright: © 2017 Attarde DL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
HPTLC technique developed as validated method for estimation of Premnine HCl in P. integrifolia chloroform extract (PI-AK) and in selected marketed formulations ‘Dashmul arishta’,‘Dashmul kadha’ in 3 × 3 batches as per ICH guidelines. Premnine HCl (Pr-s) was isolated as per literature from P. integrifolia and focused first time as standard bioactive marker for quantification. Developed mobile phase Toluene: Acetone: Diethylamine (7:2:1) for Pr-S gave Rf 0.59 at λ max 283 nm in densitometric scan, focused for specificity, fingerprint and estimation study in PI-AK and selected formulation successively. Linearity assessed in range of 8 to 16 μg /band with regression coefficient of 0.9983, LOD 0.742 μg/Band, LOQ 2.225, also robust for Pr-S. Accuracy for % recovery performed on extract as well on formulation, further subjected for precision study with application one way ANOVA for finding F value, found within limit therefore no significance of variance. A rapid and selective HPTLC method shows good linearity, recovery and high precision, useful method for analysis of Pr-S and as quality control parameter for raw material as well formulation as per foremost need of WHO, FDA and Pharmacopoeia.