Preparation and Characterization of Protein-loaded Lipid-polymer Hybrid Nanoparticles with Polycaprolactone as Polymeric Core MaterialBurcu Devrim* and Asuman BozkÄ±r
Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Tandogan, Ankara, Turkey
- *Corresponding Author:
- Burcu Devrim
Ankara University, Faculty of Pharmacy
Department of Pharmaceutical Technology
06100 TandoÄan, Ankara, Turkey
Tel: +90 312 2033162
Fax: +90 312 2131081
E-mail: [email protected]
Received date: May 19, 2014; Accepted date: June 28, 2014; Published date: June 30, 2014
Citation: Devrim B, BozkÄ±r A (2014) Preparation and Characterization of Proteinloaded Lipid-polymer Hybrid Nanoparticles with Polycaprolactone as Polymeric Core Material. J Biomol Res Ther 3:115. doi: 10.4172/2167-7956.1000115
Copyright: © Devrim B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Lipid–polymer hybrid nanoparticles (LPNs) have emerged as a potent therapeutic nano-carrier alternative to liposomes and polymeric nanoparticles. In this work, lipid–polymer hybrid nanoparticles were prepared using polycaprolactone, phosphatidylcholine: glyceryl tripalmitate mixture and lysozyme as the polymer, lipids and model protein, respectively. Uniform nanoparticles with about 100 nm in size were obtained using the modified emulsification solvent evaporation method. The results indicated that LPNs showed higher encapsulation efficiency compared with naked polycaprolactone nanoparticles. According to the results of bioactivity assay, 63.86% bioactive lysozyme was recovered from the LPNs. These results indicated that modification of polycaprolactone nanoparticles with lipids could considerably increase the drug-delivery efficiency and LPNs had potential in the delivery of peptides and proteins.