Preparation of a Novel Emulsion-Templated MIP Monolith and its Application for on Line Assay of Nifedipine in Human PlasmaGengliang Yang*, Yankun Liu, Haiyan Liu, Chunliu Yang, Ligai Bai, Mingquan Liu and Jia Cheng
Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Pharmaceutical Sciences college of Hebei University, China
- *Corresponding Author:
- Gengliang Yang
Pharmaceutical Sciences college of Hebei University
Baoding, Hebei, 071002, China
Tel: 0086 312 5971108
Fax: 0086 312 5971107
E-mail: [email protected]
Received date: September 16, 2010; Accepted date: October 28, 2010; Published date: October 30, 2010
Citation:Yang G, Liu Y, Liu H, Yang C, Bai L, et al. (2010) Preparation of a Novel Emulsion-Templated MIP Monolith and its Application for on Line Assay of Nifedipine in Human Plasma. J Chromatograph Separat Techniq 1:103. doi:10.4172/2157-7064.1000103
Copyright: © 2010 Yang G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
A novel nifedipine molecularly imprinted polymer (MIP) monolith was prepared by radical polymerization of the water in oil (W/O) emulsions, using vinyl ester resin and methacrylic acid (MAA) as the monomers and nonionic surfactants such as Polyethylene glycol 400 (PEG 400) and Pluronic F68 (PF 68) as the emulsifying agents. The properties of the material were investigated. The MIP monolithic column was installed in the high performance liquid chromatography (HPLC) system and used as the selective sorbent for on line solid-phase extraction of nifedipine in human plasma due to the good selectivity to nifedipine. For this method, the calibration curve was linear in the concentration range of 5-150 ng/mL (r2=0.998) for nifedipine in human plasma and the limit of detection (LOD) was 2 ng/mL. Accuracy, precisions and recovery was acceptable for screening nifedipine in plasma samples. The results indicated that the novel material could be used as pre-column for on line clear-up and screening of nifedipine in plasma samples, which provided a simple and rapid assay of the drugs in plasma.