Preparative Isolation and Purification of Glyceollins from Soy Bean Elicited with Aspergillus sojae by High-Speed Countercurrent Chromatography
Ojokoh Eromosele, Shi Bo*, Hujia and Liang Ping
Chinese Academy of Agricultural Science (Caas), Feed Research Institute, Zhongguancun Nandajie, Haidian, Beijing 100081, China
- *Corresponding Author:
- Shi Bo
Chinese Academy of Agricultural Science (Caas)
Feed Research Institute
Haidian, Beijing 100081, China
E-mail: [email protected]
Received date: December 20, 2011; Accepted date: February 01, 2012; Published date: February 03, 2012
Citation: Eromosele O, Bo S, Hujia, Ping L (2012) Preparative Isolation and Purification of Glyceollins from Soy Bean Elicited with Aspergillus sojae by High- Speed Countercurrent Chromatography. J Chromatograph Separat Techniq 3:119. doi:10.4172/2157-7064.1000119
Copyright: © 2012 Eromosele O, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
High-speed countercurrent chromatography (HSCCC), a support free liquid-liquid partition chromatographic technique, has been used for the preparative isolation of isoflavonoid glyceollins from the ethanol extracts of elicited soy bean seeds. The preparative HSCCC instrument is a multilayer coil planet centrifuge equipped with three preparative coils, separation was performed with a two phase solvent n-hexane/EtOAc/MeOH/H2O (3:4:2:1, v/v/v/v) at a flow rate of 1.0 mL/min in ‘head-to-tail’ mode. HSCCC separation yielded 6.0 mg of glyceollins and with purities > 86.90 %. Chemical structures of glyceollin compounds were elucidated by ESI– MS, UPLC–MS.