Preparative Separation of Lappaconitine from Aconitum leucostomum by HSCCCAmatjan A1,2, Slukhan U3, Li C1, Ke-Lin HU1 and Haji Akber Aisa1*
- *Corresponding Author:
- Haji Akber Aisa
Xinjiang Technical Institute of Physics and Chemistry
Chinese Academy of Sciences
the Key Laboratory of Xinjiang Indigenous
Medicinal Plants Resource Utilization
Urumqi 830011, China
E-mail: [email protected]
Received Date: March 14, 2017 Accepted Date: March 22, 2017 Published Date: March 27, 2017
Citation: Amatjan A, Slukhan U, Li C, Ke-Lin HU, Aisa HA (2017) Preparative Separation of Lappaconitine from Aconitum leucostomum by HSCCC. Nat Prod Chem Res 5: 258. doi: 10.4172/2329-6836.1000258
Copyright: © 2017 Amatjan A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This paper describes a system for the preparation of lappaconitine from Aconitum leucostmum Worosch., using HSCCC, and examines the effect of the two-immiscible solvent system (TISS) composed of chloroform- methanol-0.2 mol/L hydrochloric acid (4:1.5:2). Under the optimized condition, about 75 mg of lappaconitine, at a purity of 98% that was done by high performance liquid chromatography (HPLC), are obtained from 250 mg of the crude alkaloid extract. The compound was checked by HPLC-UV, electrospray ionization mass spectrometry (ESI-MS), 1H NMR and 13C NMR.