alexa Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour

Manu Chaudhary and Anurag Payasi*

Department of Cell Culture and Molecular Biology, Venus Medicine Research Centre, Baddi, Himachal Pradesh, India

*Corresponding Author:
Anurag Payasi
Venus Medicine Research Centre
Hill Top Industrial Estate
Bhatoli Kalan, Baddi, Himachal Pradesh – 173205, India
Tel: 91-1795-302068
Fax: 91-1795-302133
E-mail: [email protected]

Published date: December 11, 2013; Published date: January 10, 2014; Published date: January 15, 2014

Citation: Chaudhary M, Payasi A (2014) Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour. J Microb Biochem Technol 6:063-067. doi:10.4172/1948-5948.1000123

Copyright: © 2014 Chaudhary M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

In the present study, multi-drug resistant isolates of Escherichia coli and Pseudomonas aeruginosa were obtained from different clinical specimens and were subjected to molecular typing to detect the genes encoding oxacillinases in these isolates. Subsequently, antibacterial activity of drugs was tested against selected clinical isolates. Two hundred ninety six isolates including 98 of E. coli and 148 of P. aeruginosa were collected from clinical specimens of different centers across India. Out of 246, 123 isolates showed weak synergy for ceftazidime or cefepime and imipenem or clavulanate and were considered as oxacillinase producers. Polymerase chain reaction (PCR) was performed to identify the variants of oxacillinases genes. Our results show a great diversity of occurance of oxacillinase (OXA) genes among clinical isolates. OXA-48 and OXA-10 was more prevalent in both E. coli (32.6% OXA-48; 16.3% OXA-10) and P. aeruginosa (OXA-48 32.4%; 27.0%) as evident by PCR. The incidence of other OXA genes in E. coli and P. aeruginosa varied from 4.0 to 12.1%. Of the tested drugs, cefepime plus sulbactam was found to be the most efficacious antibacterial agents with 86.5 to 87.8% susceptibility. Cefepime plus tazobactam was second most active antibacterial agent with 72.2 to 73.5% susceptibility. Surprisingly, imipenem plus cilastatin showed susceptibility to less than 35% isolates. From the above results, it is evident that cefepime plus sulbactam has enhanced in vitro antibacterial activity compared to cefepime and imipenem plus cilastatin and tazobactam combination in oxacillinases. One significant finding of this study was that cefepime was found to be effective only against the isolates caring OXA-1 and OXA-2 but found to be resistant to OXA-10, OXA-23, OXA-24, OXA-48, OXA-51 and OXA-58 genes; whereas cefepime in combination with sulbactam was found to be the most effective against all of these OXA genes in comparison to tazobactam combination.

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