alexa Prevalence of Proteobacteria and Armatimonadetes phyla in a Photobioreactor under Carbon- and Nitrogen-Free Production Process | OMICS International| Abstract

ISSN: 2167-7972

Fermentation Technology

  • Research Article   
  • Ferment Technol 2017, Vol 6(2): 142
  • DOI: 10.4172/2167-7972.1000142

Prevalence of Proteobacteria and Armatimonadetes phyla in a Photobioreactor under Carbon- and Nitrogen-Free Production Process

Patricia Tamez-Guerra1*, Jesús O. Zuñiga-Sanchez1, Alonso A. Orozco-Flores1, Jose A. Valadez-Lira1, Cristina Rodriguez-Padilla1, Rosa O. Ca├▒izares-Villanueva2 and Ricardo Gomez-Flores1
1Department of Microbiology and Immunology, Autonomous University of Nuevo Leon, San Nicolás de los Garza, Nuevo León, Mexico
2Department of Biotechnology and Bioengineering, Center for Research and Advanced Studies of the National Polytechnic Institute, Instituto Politécnico Nacional, San Pedro Zacatenco, Mexico
*Corresponding Author : Patricia Tamez-Guerra, Department of Microbiology and Immunology, Autonomous University of Nuevo Leon, San Nicolás De Los Garza, Mexico, Tel: +0115281-83298-4000 , Email: [email protected]

Received Date: Mar 29, 2017 / Accepted Date: Apr 25, 2017 / Published Date: May 02, 2017

Abstract

In prokaryotes, the autotrophic population within the bacteria kingdom presents great biotechnological potential. For taxonomic analysis of species, the sequence encoding the small subunit ribosomal RNA gene (16S rRNA) is currently the most reliable technique for phylogenetic bacterial classification. Sequencing can define the population diversity and ecological importance within a habitat. The study of autotrophic bacteria population dynamics in a photobioreactor using a carbon and nitrogen free culture medium could provide information about succession dynamics under controlled conditions. The aim of this study was to identify the bacterial populations present in a photobioreactor by 16S rRNA amplicon sequence comparison from samples taken at 0, 7, and 14 fermentation days. Most of the identified clones were described to the level of genera from the following phyla: Proteobacteria (40%), Armatimonadetes (35%), Firmicutes (17.5%), Actinobacteria (2.5%), and Cyanobacteria/Chloroplast (2.5%). Bacterial strains from those phyla have been reported in aquatic environments and were considered uncultivable; however, sequences indicated the presence of the clones A-123 (ID: HQ860522.1) from time 0, and YHS25 (GU305825.1) after 7 days of culture. Our findings demonstrated the major presence of autotrophic bacterial populations of the Proteobacteria and Armatimonadetes phyla, which grew under artificial culture conditions in a photobioreactor.

Keywords: Prokaryotes; Autotroph population; Biotechnology; Bacterial adaptation; Aquatic environments

Citation: Guerra P, Sanchez JZ, Flores AO, Lira JV, Padilla CR, et al. (2017) Prevalence of Proteobacteria and Armatimonadetes phyla in a Photobioreactor under Carbon and Nitrogen-Free Production Process. Ferment Technol 6: 142. Doi: 10.4172/2167-7972.1000142

Copyright: © 2017 Guerra P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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