Preventive Action of Honey on Methotrexate Induced Intestinal Mucositis in Albino Rats (Immunohistochemical Study)Ali Sultan Al-Refai *
Anatomy Branch - Department of Oral and Maxillofacial Surgery, College of Dentistry, Hawler Medical University, Erbil, Kurdistan Region of Iraq, Iraq
- *Corresponding Author:
- Ali Sultan Al-Refai
Anatomy Branch - Department of Oral & Maxillofacial Surgery
College of Dentistry, Hawler Medical University
Erbil, Kurdistan Region of Iraq, Iraq
E-mail: [email protected]
Received Date: June 16, 2014; Accepted Date: July 18, 2014; Published Date: July 20, 2014
Citation: Al-Refai AS (2014) Preventive Action of Honey on Methotrexate Induced Intestinal Mucositis in Albino Rats (Immunohistochemical Study). J Cytol Histol 5:265. doi:10.4172/2157-7099.1000265
Copyright: © 2014 Al-Refai AS. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background and Objectives: Gastrointestinal mucositis is a severe and debilitating side effect of chemotherapy. Methotrexate (MTX) represents one of the most potent classical anti-tumor drugs. The aim of this study was to determine the preventive effects of honey on the intestinal mucositis caused by methotrexate in rats.
Materials and Methods: In current study forty females Albino rats, weighing 250-300g were used in the study. For the induction of mucositis, 60 mg/kg of MTX was administered intraperitoneally to each animal in the study group on day 4. The control animals were intraperitoneally injected by normal saline in the same manner and dose like MTX. At the beginning of the experiment, the rats in each group were randomly divided into two groups: Distilled water treated group and honey treated group (10 animals each). A volume of distilled water equal to honey was given by intragastric gavage tube, while the other group was gavaged with honey at a dose of (2.5g/kg) two times daily. The body weight for each rat was measured and then the animals were sacrificed on day 8. In each experiment, one centimeter of prox¬imal jejunum was removed for histopathological, intestinal morphometry, and immunohistochemical analysis using Ki-67 and Bcl-2 immunolabeling.
Results: The MTX / honey treated group showed significantly (p<0.05) less body weight loss and less histopathological scores than that of MTX / water treated group. But there were non significant differences (p>0.05) were seen in the Ki-67 and Bcl-2 immune expression in comparison with the MTX/water treated group.
Conclusion: Honey at a dose of 2.5g/kg two times daily, produced a partial protection against methotrexate induced mucositis in rat by decreasing the histopathological scores.