alexa Process Optimization of L-Glutaminase Production; a Tumour Inhibitor from Marine Endophytic Isolate Aspergillus sp. ALAA-2000
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Process Optimization of L-Glutaminase Production; a Tumour Inhibitor from Marine Endophytic Isolate Aspergillus sp. ALAA-2000

Mervat Morsy Abbas Ahmed1,2*, Taher M Taha3,4, Nageh F Abo-Dahab3 and Fareed SM Hassan3

1Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University (KAU), Saudi Arabia

2Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt

3Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assuit, Egypt

4Biology Department, Faculty of Science and Arts, Al Baha University, Saudi Arabia

*Corresponding Author:
Mervat Morsy Abbas Ahmed
Department of Biological Sciences
Faculty of Sciences
King Abdulaziz University (KAU)
Saudi Arabia
Tel: 0000-0002-0680-3205
E-mail: [email protected]

Received Date: July 03, 2016; Accepted Date: July 29, 2016; Published Date: August 09, 2016

Citation: Ahmed MMA, Taha TM, Abo-Dahab NF, Hassan FSM (2016) Process Optimization of L-Glutaminase Production; a Tumour Inhibitor from Marine Endophytic Isolate Aspergillus sp. ALAA-2000. J Microb Biochem Technol 8: 382- 389. doi: 10.4172/1948-5948.1000313

Copyright: © 2016 Abd-El-Karem Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

L-Glutaminases have received significant attention recently owing to their potential applications. All endophytic fungi recovered from the marine soft sponge Aplysina fistularis were able to produce L-glutaminase. During screening program, Aspergillus sp. ALAA-2000 showed the highest L-glutaminase production levels. The production of L-glutaminase by Aspergillus sp. ALAA-2000 was evaluated under different fermentation modes and parameters. The L-glutaminase synthesis was increased their yield after the optimization of fermentation parameters. The hot water 40°C was the best leaching agent extracted of soy bean for L-glutaminase production (21.89 U/ml) under solid state fermentation (SSF). The highest L-glutaminase activity (91.92 U/ml) was achieved after two days incubation period under submerged fermentation (SmF). L-glutamine, dextrose, cysteine and Magnesium chloride supported the highest L-glutaminase production by Aspergillus sp. ALAA-2000 under SmF at pH 4 and 27°C. Single peak of L-glutaminase was obtained from the culture supernatant of Aspergillus sp. ALAA-2000 through ammonium sulfate precipitation and DEAE-cellulose column chromatographyrefer to the mono meric nature of L-glutaminase enzyme. The parameters of purified L-glutaminase were optimized as follow: pH 10, stable at 40°C to 50°C, reaction time 30 min and substrate concentration 4.38 mg/ml. Whereas the maximum activator cation is Na+ and different EDTA concentrations have no effect on L-glutaminase activity which means that L-glutaminase enzymes was represent as a non-metallic enzyme.

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