alexa Production and Characterization of Polyhydroxybutryrate
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Production and Characterization of Polyhydroxybutryrate Biopolymer from Azohydromonas australica Using Sucrose as a Sole Carbon Source

Salman Ahmad1, Abuzer Amir2, Md. Zafaryab3, Khwaja Osama1, Soban Ahmad Faridi1, Mohammed Haris Siddiqui1, Moshahid A Rizvi M3 and Md. Asad Khan4*

1Department of Bioengineering, Integral University, India

2Shri Ram Swaroop Memorial University, India

3Department of Biosciences, Jamia Millia Islamia, India

4Department of Dentistry, Jamia Millia Islamia, India

*Corresponding Author:
Md. Asad K
Faculty of Dentistry, Jamia Millia Islamia
New Delhi, India
Tel: 01126981717
E-mail: [email protected]

Received date: April 29, 2017; Accepted date: May 15, 2017; Published date: May 22, 2017

Citation: Ahmad S, Amir A, Zafaryab M, Osama K, Faridi SA, et al. (2017) Production and Characterization of Polyhydroxybutryrate Biopolymer from Azohydromonas australica Using Sucrose as a Sole Carbon Source. J Microb Biochem Technol 9:082-086. doi:10.4172/1948-5948.1000348

Copyright: © 2017 Ahmad S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A bacterial strain Azohydromonas australica DSM 1124 has been chosen, which is accumulated intracellular poly- β-hydroxybutyrate particles, each method which has been merit and demerit for employed in PHB extraction. Our study, we have selected chloroform-sodium hypochlorite method. It is one of the methods for extracting PHB forms Azohydromonas australica DSM 1124. Polyhydroxybutyrate is a biodegradable and biocompatible thermoplastic with many interesting applications in medicine, food packaging and tissue engineering materials. That does not produce any toxins or residues in the environment like petroleum based plastics. The present study was emphasized on enhanced production of PHB by Azohydromonas australica using sucrose as a sole carbon source and estimation of biomass and sucrose content in the media.The batch kinetics analysis of Azohydromonas australica was done an interval of 3 h. Batch cultivation with optimized media recipe in a 7 L bioreactor exhibited a maximum biomass 1.71 g/L and PHB concentration 2.67 g/L for A. australica. The characterization of PHB was done by growth kinetics studies, UV-Spectrophotometer and Fourier transforms infrared spectroscopy (FTIR).

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