alexa Production and Optimization of Lipase Enzyme from Mesop
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Production and Optimization of Lipase Enzyme from Mesophiles and Thermophiles

Jaiswal A, Preet M and Tripti B*

Codon Biotech Pvt. Ltd., India

Corresponding Author:
Tripti B
Codon Biotech Pvt. Ltd., India
Tel: 01204311464
E-mail: [email protected]

Received date: November 30, 2016; Accepted date: June 06, 2017; Published date: June 13, 2017

Citation: Jaiswal A, Preet M, Tripti B (2017) Production and Optimization of Lipase Enzyme from Mesophiles and Thermophiles. J Microb Biochem Technol 9:126-131. doi:10.4172/1948-5948.1000355

Copyright: © 2017 Jaiswal A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Lipases are enzymes which catalyze the hydrolysis of triacylglycerol to free fatty acids and glycerol. In the present study bacterial cultures were isolated from industrial and oil spilled areas and was screened for Lipase production and activity. Seven bacterial strains were found to have lipolytic ability. These bacterial strains were grown in production media and the lipase enzyme produced was estimated. After optimization of factors like pH, temperature, carbon source, nitrogen and incubation time etc. a maximum lipase enzyme activity of 8U was obtained in the mesophilic bacteria: P. mirabilis when sunflower oil was used as a substrate, pH was 6 and temperature was 37°C. B. coagulans, another mesophile produced 7.5U of lipase enzyme. Thermophiles: P. stutzeri, G. stearothermophilus and B. sporothermodurans which were isolated, screened for lipase activity and characterized, showed highest lipase activity of 7U at 50°C.

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