alexa Production of L-Aparaginase by Using <em>Pectobacterium carotovorum</em> | OMICS International| Abstract

ISSN: 2329-8901

Journal of Probiotics & Health

  • Research Article   
  • J Prob Health 2017, Vol 5(1): 168
  • DOI: 10.4172/2329-8901.1000168

Production of L-Aparaginase by Using Pectobacterium carotovorum

Jigeesha Jetti1*, Aravind Jetti2 and Raju Perla3
1Department of Microbiology, SVU Collge of Sciences Campus, Sri Venkateswara University, Tirupati, Andhra Pradesh, India
2Centre for Biotechnology, Department of Chemical Engineering, College of Engineering(A), Andhra University, Visakhapatnam, Andhra Pradesh, India
3Department of Biotechnology, Godavari Institite of Engineering and Technology, Chaitanya Knowledge City, Rajahmundry, Andhra Pradesh, India
*Corresponding Author : Jigeesha Jetti, Department of Microbiology, SVU Collge of Sciences Campus, Sri Venkateswara University, Tirupati, Andhra Pradesh, India, Tel: 9492391218, Email: [email protected]

Received Date: Mar 04, 2017 / Accepted Date: Mar 16, 2017 / Published Date: Mar 27, 2017


L-Asparaginase appears to be highly effective especially in children with newly diagnosed Acute lymphoblastic leukemia. Prolonged L-Asparaginase intensification improved the outcome significantly. Nowadays L-Asparaginase is an essential drug that is used to treat children with Acute lymphoblastic leukemia all over the world. Its potential is now well established, as it has remarkably induced remission in most of the patients suffering with this disease. This therapy has brought a major breakthrough in modern oncology and with the development of its new functions, a great demand for L-Asparaginase is expected in the coming years. Keeping the potential of this particular enzyme in cancer treatment, the present work is planned with an intention of optimizing the production of L-Asparaginase both by conventional as well as by genetic engineering techniques. Pectobacterium carotovorum is selected for the present work based on its potentiality in cancer treatment and due to less glutaminase activity compared to the enzyme sourced from E. coli. Microbial culture was procured from MTCC, Chandigarh and screened for L-Asparaginase production in plate method and identified the activity based on the formation of pink color zone after overnight incubation.

Keywords: L-Asparaginase; Lymphoblastic leukemia; Pectobacterium carotovorum; Enzyme activity

Citation: Jetti J, Jetti A, Perla R (2017) Production of L-Aparaginase by Using Pectobacterium carotovorum. J Prob Health 5: 168. Doi: 10.4172/2329-8901.1000168

Copyright: © 2017 Aravind J. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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