alexa Production of Microbial Lipids from Rice Straw Hydrolys
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Production of Microbial Lipids from Rice Straw Hydrolysates by Lipomyces starkeyi for Biodiesel Synthesis

Abul Kalam Azad1*, Abu Yousuf2, Atia Ferdoush1, Md. Mahbub Hasan1, Md. Rezaul Karim3 and Asraful Jahan1

1Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh

2Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh

3Oilseed and Lipid Technology Section, Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh

*Corresponding Author:
Abul Kalam Azad
Department of Genetic Engineering and Biotechnology
Shahjalal University of Science and Technology
Sylhet 3114, Bangladesh
Tel: +88-0821-717850 Ex411
Fax: +88-0821-725050
E-mail: [email protected]

Published date: September 29, 2014; Published date: October 19, 2014; Published date: October 27, 2014

Citation: Azad AK, Yousuf A, Ferdoush A, Mahbub Hasan Md, Rezaul Karim Md, et al. (2014) Production of Microbial Lipids from Rice Straw Hydrolysates by Lipomyces starkeyi for Biodiesel Synthesis. J Microb Biochem Technol S8:008. doi:10.4172/1948-5948.S8-008

Copyright: © 2014 Azad AK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author andsource are credited

 

Abstract

To minimize the cost of biodiesel production, massively available biomass, rice straw was selected as starting material for fermentation by oleaginous yeast, Lipomyces starkeyi. Acid hydrolysis was carried out to obtain Rice Straw Hydrolysates (RSH). Maximum amount of glucose was liberated with 3.5% H2SO4. Highest levels of biomass (~12 g/L) and lipid content (~36%) of L. starkeyi were obtained through fermentation of RSH produced with 3.5% H2SO4. The optimum pH for biomass yield and lipid accumulation of L. starkeyi was 6.0. Periodic supplementation of fresh RSH increased lipid content and lipid yield approximately15% and 40%, respectively. The growth and lipid accumulation of L. starkeyi were enhanced when carbon and nitrogen sources were supplemented with RSH. Gas chromatographic analysis revealed that the lipid obtained from L. starkeyi cultivated with RSH was composed of saturated and unsaturated fatty acids. The fatty acid composition of this microbial lipid is similar to that of vegetable oils. The results reported herein indicate that rice straw might be a valuable alternative feedstock for microbial lipids production by L. starkeyi for biodiesel synthesis.

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