PMC/PubMed Indexed Articles
Indexed In
- Academic Journals Database
- Genamics JournalSeek
- Academic Keys
- JournalTOCs
- China National Knowledge Infrastructure (CNKI)
- Scimago
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Directory of Research Journal Indexing (DRJI)
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- MIAR
- University Grants Commission
- Geneva Foundation for Medical Education and Research
- Euro Pub
- Google Scholar
Useful Links
Share This Page
Journal Flyer
Open Access Journals
- Agri and Aquaculture
- Biochemistry
- Bioinformatics & Systems Biology
- Business & Management
- Chemistry
- Clinical Sciences
- Engineering
- Food & Nutrition
- General Science
- Genetics & Molecular Biology
- Immunology & Microbiology
- Medical Sciences
- Neuroscience & Psychology
- Nursing & Health Care
- Pharmaceutical Sciences
Abstract
Production of Recombinant Heat Shock Protein 60 (HSP60) From Salmonella enterica serovar Typhimurium ATCC 19585 and Its Evaluation as a Vaccine Candidate in BALB/c Mice
Israa F Saied, Ghazi M Aziz, Ali H Ad\'hiah and A Mahdi Saeed
The gene for heat shock protein 60 (HSP60) was amplified from the DNA extract of Salmonella enterica serovar Typhimurium strain (LT2), ATCC 19585, using Polymerase Chain Reaction (PCR). The HSP60 gene was partially sequenced, inserted into expression vector and cloned into a competent Escherichia coli. The expressed recombinant HSP60 protein was purified by Ni-NTA affinity chromatography. Immunization of BALB/c mice with the purified recombinant HSP60 protein resulted in a significant anti-HSP60 antibody titers. The groups of the immunized and control mice were challenged with lethal doses of S. Typhimurium (LT2) ATCC 19585. Immunized mice had a higher survival rate than control mice suggesting the protective value of the recombinant HSP60 protein used for vaccination.