alexa Production of Recombinant Heat Shock Protein 60 (HSP60)
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Production of Recombinant Heat Shock Protein 60 (HSP60) From Salmonella enterica serovar Typhimurium ATCC 19585 and Its Evaluation as a Vaccine Candidate in BALB/c Mice

Israa F Saied1, Ghazi M Aziz1, Ali H Ad'hiah1 and A Mahdi Saeed2*

1Department of Biotechnology, College of Science, University of Baghdad, Jadriyah, Baghdad, Iraq

2Departments of Large Animal Clinical Sciences and Epidemiology, Michigan State University, USA

*Corresponding Author:
A Mahdi Saeed
Departments of Large Animal Clinical Sciences and Epidemiology
Michigan State University, East Lansing
Michigan 48824, USA
Tel: +15174329517
Fax: +15174322310
E-mail: [email protected]

Published date: July 03, 2014; Published date: August 22, 2014; Published date: August 29, 2014

Citation: Saied IF, Aziz GM, Ad'hiah AH, Mahdi Saeed A (2014) Production of Recombinant Heat Shock Protein 60 (HSP60) From Salmonella enterica serovar Typhimurium ATCC 19585 and Its Evaluation as a Vaccine Candidate in BALB/c Mice. J Microb Biochem Technol 6:346-350. doi:10.4172/1948-5948.1000166

Copyright: © 2014 Saied IF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

The gene for heat shock protein 60 (HSP60) was amplified from the DNA extract of Salmonella enterica serovar Typhimurium strain (LT2), ATCC 19585, using Polymerase Chain Reaction (PCR). The HSP60 gene was partially sequenced, inserted into expression vector and cloned into a competent Escherichia coli. The expressed recombinant HSP60 protein was purified by Ni-NTA affinity chromatography. Immunization of BALB/c mice with the purified recombinant HSP60 protein resulted in a significant anti-HSP60 antibody titers. The groups of the immunized and control mice were challenged with lethal doses of S. Typhimurium (LT2) ATCC 19585. Immunized mice had a higher survival rate than control mice suggesting the protective value of the recombinant HSP60 protein used for vaccination.

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