alexa Prolactin I Microsatellite as Genetic Markers for Chara
ISSN: 2155-9546

Journal of Aquaculture Research & Development
Open Access

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Research Article

Prolactin I Microsatellite as Genetic Markers for Characterization of Five Oreochromis Tilapia Species and Two Oreochromis Niloticus Strains

Jing Ruei Chi1,2, Chang-Wen Huang2, Jen Leih Wu2 and Shao Yang Hu3*
1Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan
2Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
3Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan
Corresponding Author : Shao-Yang Hu, MD
Department of Biological Science and Technology
National Pingtung University of Science and Technology
No. 1, Hseufu Road, Pingtung, Taiwan
Tel: 886-8-7703202
E-mail: [email protected]
Received May 20, 2014; Accepted July 24, 2014; Published August 03, 2014
Citation: Chi JR, Huang CW, Wu JL, Hu SY (2014) Prolactin I Microsatellite as Genetic Markers for Characterization of Five Oreochromis Tilapia Species and Two Oreochromis Niloticus Strains. J Aquac Res Development 5:251. doi: 10.4172/2155-9546.1000251
Copyright: © 2014 Chi JR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Hybrid tilapia is a major aquaculture food fish species in developing countries. The development of molecular
markers to characterize and trace tilapia species is necessary to improve tilapia quality and enhance the competitive advantage of the aquaculture industry. Microsatellite markers have been suggested to assist breeding, species identification, and traceability system for tilapia. We used six microsatellites markers located within growth-related genes to discriminate between several tilapia species. We found that a combination of two microsatellites markers located within the proximal promoter of the prolactin I (PRL I) gene, PRL I-MS01 and PRL I-MS02, were able to discriminate between five Oreochromis tilapia species (O. mossambicus, O. aureus, O. niloticus, O. hornorum and O. spilurus) and two O. niloticus strains that exhibit the distinctive growth traits. Furthermore, we found that PRL I-MS01 microsatellite marker was able to trace parental origin of hybrid tilapia. Thus, this marker is a potentially beneficial tool for a tilapia traceability system. We conclude that the GT tandem repeats in PRL I-MS01 and CA tandem repeats
in PRL I-MS02 are useful genetic markers to characterize diverse tilapia species, assist in the genetic tracing and conventional breeding of superior strains and strengthen the management of the tilapia aquaculture industry. There was strong relationship between the cold and both temperature at death and cumulative degree hours represented by negative correlation coefficient for all tested fish. There was no correlation between cold tolerance and fish size for all tested fish. The cooling degree hours were significantly different between the selected and non-selected O. niloticus (P<0.005). The selected O. aureus exhibited greater cold tolerance than the non-selected and death began at 14.1°C, while non-selected occurred at 15.2°C.

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