alexa Proliferative Nodules in Congenital Nevi - A Histopathologic, Genetic and Immunohistochemical Reappraisal
ISSN: 2155-9554

Journal of Clinical & Experimental Dermatology Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Proliferative Nodules in Congenital Nevi - A Histopathologic, Genetic and Immunohistochemical Reappraisal

Meera Brahmbhatt1, Shi Yang2 and Meera Mahalingam3*

1Sargent College, Boston University, USA

2Departments of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston MA, USA

3Dermatopathology Section, Department of Dermatology, Boston University School of Medicine, Boston MA, USA

*Corresponding Author:
Meera Mahalingam
Dermatopathology Section
Department of Dermatology
Boston University School of Medicine
609 Albany Street, J-301
Boston, MA 02118, USA
Tel: 617 638 5574
Fax: 617 638 5575
Email: [email protected]

Received date: November 11, 2010; Accepted date: December 06, 2010; Published date: December 07, 2010

Citation: Brahmbhatt M, Yang S, Mahalingam M (2010) Proliferative Nodules in Congenital Nevi - A Histopathologic, Genetic and Immunohistochemical Reappraisal. J Clin Exp Dermatol Res 1:105. doi: 10.4172/2155-9554.1000105

Copyright: © 2010 Brahmbhatt M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.



Although histopathology is the gold standard for the classification of melanocytic proliferations, in some such as the proliferative nodule, an equivocal diagnosis can be diagnostically extremely challenging based on histopathologic features alone. The purpose of this study was to review the histologic features of proliferative nodules and to ascertain the utility of immunohistochemistry and analyses of oncogenic mutations in signaling components of the MAP kinase pathway as diagnostic adjuncts. Genomic DNA for genotyping was isolated per protocol using techniques that included laser capture microdissection to isolate nevus cells from proliferative nodules (n=3) and age-matched congenital nevi (CN, n=3). Direct DNA sequencing was performed on BRAF codon 600; NRAS1 codons 12 and 13; NRAS2 codons 60 and 61, KRAS codons 12 and 13 and GNAQ. Immunohistochemical analyses were performed using antibodies to nestin, CD133, p53, c-kit and bcl-2. While all 3 cases of proliferative nodules exhibited mitoses, features of concern were not noted in any. Of the genes analyzed, no mutations were identified in any of the PN. Immunohistochemistry revealed the following: nestin in 1 PN (3+) and in 2 CN (both 3+); CD133 negative in all PN and CN; p53 in 1 PN (2+) and 0 in CN; c-kit in 2 PN (2+/3+) and in 3 CN (2+/3+/3+) and bcl-2 in 2 PN (both 3+) and 2 CN (both 3+). Our findings, albeit limited by sample size, suggest that proliferative nodules do not appear to possess a distinctive or unifying genomic signature. In light of evidence indicating that progression to malignant melanoma involves genetic pathways instrumental to stem cell biology, that absence of a sizeable population of stem cells in 2 of 3 of proliferative nodules in the current study supports their putative benign biologic behavior.

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version