Promoting and Harmful Effects of Steroid Hormones on Renal Stem/Progenitor Cell DevelopmentWill W. Minuth*, Lucia Denk and Anne Glashauser
Molecular and Cellular Anatomy, University of Regensburg, University Street 31, D-93053 Regensburg, Germany
- Corresponding Author:
- Dr. Will W. Minuth
Molecular and Cellular Anatomy
University of Regensburg, University Street 31
D-93053 Regensburg, Germany
Tel: +49 (0)941 943 2876
Fax: +49 (0)941 943 2868
E-mail: [email protected]
Received date: October 11, 2010; Accepted date: October 23, 2010; Published date: November 06, 2010
Citation: Minuth WW, Denk L, Glashauser A (2010) Promoting and Harmful Effects of Steroid Hormones on Renal Stem/Progenitor Cell Development. J Tissue Sci Eng 1:101. doi:10.4172/2157-7552.1000101
Copyright: © 2010 Minuth WW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
In regenerative medicine much attention is given to stem/progenitor cells for a future therapy of acute and chronic renal failure. However, up to date sound cell biological knowledge about nephron renewal within the diseased kidney is lacking. Of special interest are steroid hormones occurring in the interstitial fluid. It is unknown, if they have promoting or hampering effects on the development of stem/progenitor cells. To investigate the influence of steroid hormones on regenerating tubules a realistic culture system is of great importance. An advanced technique creates an artificial interstitium promoting renal stem/progenitor cells to develop tubules between layers of polyester fleece. During perfusion culture with chemically defined Iscove’s Modified Dulbecco’s Medium (IMDM) individual steroid hormones can be administered. After a culture period of 13 days the effect on tubule development is analyzed by histochemical methods. Present experiments reveal that substances such as cholesterol and 17-α-hydroxyprogesterone do not exhibit a visible effect on renal stem/progenitor cells. However, atypical aggregation of few SBA-labeled cells in form of islets is found after treatment with progesterone, pregnenolone, 11-deoxycorticosterone and dihydrotestosterone. Numerous SBA-labeled cells within extended clusters are observed after application of testosterone, 17-β-estradiol, corticosterone and dexamethasone. In contrast, application of aldosterone exclusively leads to the formation of numerous intact tubules. In consequence, it is shown that distinct steroid hormones induce a shift in development from intact tubules to atypical cell islets and clusters. Such harmful effects have to be considered, when stem/progenitor cells are exposed after implantation to the interstitial fluid within diseased renal parenchyma.